mRNA encoding bone morphogenetic protein-2 facilitated peri-implant bone formation of titanium implants placed in rat femurs
Abstract This study evaluated the effectiveness of N1-methylpseudouridine-modified mRNA encoding bone morphogenetic protein-2 (BMP-2 mRNA-LNP) in promoting bone growth and improving titanium implant integration in rat femur bone defects. Sixteen Sprague-Dawley rats (32 femurs) were used, with titani...
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Nature Portfolio
2025-05-01
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| Online Access: | https://doi.org/10.1038/s41598-025-02931-x |
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| author | Kanoksiri Jriyasetapong Nopparada Lawtrakulngam Philaiporn Vivatbutsiri Sunporn Namano Kamolporn Wattanasirmkit Somphote Angkhanawiriyarak Jaijam Suwanwela |
| author_facet | Kanoksiri Jriyasetapong Nopparada Lawtrakulngam Philaiporn Vivatbutsiri Sunporn Namano Kamolporn Wattanasirmkit Somphote Angkhanawiriyarak Jaijam Suwanwela |
| author_sort | Kanoksiri Jriyasetapong |
| collection | DOAJ |
| description | Abstract This study evaluated the effectiveness of N1-methylpseudouridine-modified mRNA encoding bone morphogenetic protein-2 (BMP-2 mRNA-LNP) in promoting bone growth and improving titanium implant integration in rat femur bone defects. Sixteen Sprague-Dawley rats (32 femurs) were used, with titanium wires implanted in the femoral defects. The defects were divided into four groups (n = 8): 5 µg BMP-2 mRNA-LNP, 15 µg BMP-2 mRNA-LNP, recombinant BMP-2 (rhBMP-2), and Dulbecco’s phosphate-buffered saline (dPBS). Following euthanasia at 3 and 6 weeks, Micro-Computed Tomography (micro-CT) was performed to assess bone volume, trabecular architecture, and bone-to-implant contact. Push-out mechanical testing determined the maximal loading force for implant dislodgment, while elemental analysis using Energy-Dispersive X-ray Spectroscopy (EDS) and Scanning Electron Microscopy (SEM) assessed mineralization on the titanium surfaces. The 15 µg BMP-2 mRNA-LNP group demonstrated significantly enhanced bone volume, trabecular thickness, and bone-to-implant contact compared to the control group (p < 0.05). Additionally, this group showed higher calcium and phosphorus content, indicating superior mineralization. Concomitantly, the mean maximal loading force (N) increased in the BMP-2 mRNA-LNP group, though this increase was not statistically significant. These results suggest that BMP-2 mRNA-LNP offers a promising approach to enhance peri-implant bone formation and bone regeneration. While the 15 µg group showed slightly greater bone formation, most experimental parameters showed no significant differences compared to the 5 µg group, suggesting the lower dose may offer a more cost-effective approach without compromising efficacy. |
| format | Article |
| id | doaj-art-b1b0bc249f464261bfe0c79d70fe037e |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-b1b0bc249f464261bfe0c79d70fe037e2025-08-20T03:48:19ZengNature PortfolioScientific Reports2045-23222025-05-0115111210.1038/s41598-025-02931-xmRNA encoding bone morphogenetic protein-2 facilitated peri-implant bone formation of titanium implants placed in rat femursKanoksiri Jriyasetapong0Nopparada Lawtrakulngam1Philaiporn Vivatbutsiri2Sunporn Namano3Kamolporn Wattanasirmkit4Somphote Angkhanawiriyarak5Jaijam Suwanwela6Department of Prosthodontics, Faculty of Dentistry, Chulalongkorn UniversityDepartment of Prosthodontics, Faculty of Dentistry, Chulalongkorn UniversityDepartment of Anatomy, Faculty of Dentistry, Chulalongkorn UniversityImplant and Esthetic Dentistry Program, Faculty of Dentistry, Chulalongkorn UniversityDepartment of Prosthodontics, Faculty of Dentistry, Chulalongkorn UniversityImplant and Esthetic Dentistry Program, Faculty of Dentistry, Chulalongkorn UniversityDepartment of Prosthodontics, Faculty of Dentistry, Chulalongkorn UniversityAbstract This study evaluated the effectiveness of N1-methylpseudouridine-modified mRNA encoding bone morphogenetic protein-2 (BMP-2 mRNA-LNP) in promoting bone growth and improving titanium implant integration in rat femur bone defects. Sixteen Sprague-Dawley rats (32 femurs) were used, with titanium wires implanted in the femoral defects. The defects were divided into four groups (n = 8): 5 µg BMP-2 mRNA-LNP, 15 µg BMP-2 mRNA-LNP, recombinant BMP-2 (rhBMP-2), and Dulbecco’s phosphate-buffered saline (dPBS). Following euthanasia at 3 and 6 weeks, Micro-Computed Tomography (micro-CT) was performed to assess bone volume, trabecular architecture, and bone-to-implant contact. Push-out mechanical testing determined the maximal loading force for implant dislodgment, while elemental analysis using Energy-Dispersive X-ray Spectroscopy (EDS) and Scanning Electron Microscopy (SEM) assessed mineralization on the titanium surfaces. The 15 µg BMP-2 mRNA-LNP group demonstrated significantly enhanced bone volume, trabecular thickness, and bone-to-implant contact compared to the control group (p < 0.05). Additionally, this group showed higher calcium and phosphorus content, indicating superior mineralization. Concomitantly, the mean maximal loading force (N) increased in the BMP-2 mRNA-LNP group, though this increase was not statistically significant. These results suggest that BMP-2 mRNA-LNP offers a promising approach to enhance peri-implant bone formation and bone regeneration. While the 15 µg group showed slightly greater bone formation, most experimental parameters showed no significant differences compared to the 5 µg group, suggesting the lower dose may offer a more cost-effective approach without compromising efficacy.https://doi.org/10.1038/s41598-025-02931-xBone morphogenetic Protein-2 (BMP-2)Dental implantBone regenerationPeri-implant bone formationmRNAPreclinical studies |
| spellingShingle | Kanoksiri Jriyasetapong Nopparada Lawtrakulngam Philaiporn Vivatbutsiri Sunporn Namano Kamolporn Wattanasirmkit Somphote Angkhanawiriyarak Jaijam Suwanwela mRNA encoding bone morphogenetic protein-2 facilitated peri-implant bone formation of titanium implants placed in rat femurs Scientific Reports Bone morphogenetic Protein-2 (BMP-2) Dental implant Bone regeneration Peri-implant bone formation mRNA Preclinical studies |
| title | mRNA encoding bone morphogenetic protein-2 facilitated peri-implant bone formation of titanium implants placed in rat femurs |
| title_full | mRNA encoding bone morphogenetic protein-2 facilitated peri-implant bone formation of titanium implants placed in rat femurs |
| title_fullStr | mRNA encoding bone morphogenetic protein-2 facilitated peri-implant bone formation of titanium implants placed in rat femurs |
| title_full_unstemmed | mRNA encoding bone morphogenetic protein-2 facilitated peri-implant bone formation of titanium implants placed in rat femurs |
| title_short | mRNA encoding bone morphogenetic protein-2 facilitated peri-implant bone formation of titanium implants placed in rat femurs |
| title_sort | mrna encoding bone morphogenetic protein 2 facilitated peri implant bone formation of titanium implants placed in rat femurs |
| topic | Bone morphogenetic Protein-2 (BMP-2) Dental implant Bone regeneration Peri-implant bone formation mRNA Preclinical studies |
| url | https://doi.org/10.1038/s41598-025-02931-x |
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