Self-assembled injectable Icariin@ Ti3C2Tx/doxorubicin hydrogel preserving osteogenesis while synergizing photodynamic and chemodynamic therapy for osteosarcoma
Abstract Local therapy involving injectable hydrogel systems loaded with doxorubicin (DOX) has garnered significant attention in the realm of osteosarcoma (OS) research. Nevertheless, it has been noted that the local delivery of high-dose DOX exerts a pronounced inhibitory impact on osteogenesis, wh...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer
2025-03-01
|
| Series: | Journal of Materials Science: Materials in Medicine |
| Online Access: | https://doi.org/10.1007/s10856-025-06874-7 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850039404722126848 |
|---|---|
| author | Jiahao Fu Hao Chen Yiqiao Zhao Hongzhong Xi Yixuan Huang Chenglin Liu Yaokun Wu Wei Song Xin Liu Bin Du Guangquan Sun |
| author_facet | Jiahao Fu Hao Chen Yiqiao Zhao Hongzhong Xi Yixuan Huang Chenglin Liu Yaokun Wu Wei Song Xin Liu Bin Du Guangquan Sun |
| author_sort | Jiahao Fu |
| collection | DOAJ |
| description | Abstract Local therapy involving injectable hydrogel systems loaded with doxorubicin (DOX) has garnered significant attention in the realm of osteosarcoma (OS) research. Nevertheless, it has been noted that the local delivery of high-dose DOX exerts a pronounced inhibitory impact on osteogenesis, which is detrimental to the restoration of functional capabilities after OS treatment. To address this challenge, we have designed a self-assembled injectable hydrogel system that integrates photodynamic and chemodynamic therapy, aiming to enhance efficacy while mitigating adverse effects on osteogenic differentiation. In this study, an injectable sodium alginate (SA) hydrogel was fabricated by encapsulating titanium carbide powder (Ti3C2Tx) and osteoprotegerin Icariin (ICA) along with DOX. This hydrogel system demonstrated remarkable drug-loading capacity and sustained drug release. Furthermore, under near-infrared (NIR) irradiation, the hydrogel displayed outstanding photothermal effects, which, in conjunction with chemotherapy and phototherapy, effectively eradicated UMR-106 tumor cells in vitro. The incorporation of ICA not only enhanced the anti-tumor effect but also alleviated the adverse effects of DOX on the osteogenic differentiation inhibition of bone marrow mesenchymal stem cells (BMSCs). In vivo, findings further confirmed that injectable ITD/SA hydrogels can synergistically heighten anti-osteosarcoma effectiveness while mitigating local osteogenic toxicity. Given these benefits, this hydrogel holds extensive application prospects in the local therapy of OS. Graphical Abstract Schematic diagram of injectable NIR-responsive ITD/SA hydrogel phototherapy versus chemotherapy for OS and protection against BMSCS. (A) Illustration of the preparation process of the ITD/SA hydrogel. (B) Schematic of the therapeutic effect of ITD/SA under NIR irradiation. |
| format | Article |
| id | doaj-art-b19d4f9665854e548c49b8a60ee5c862 |
| institution | DOAJ |
| issn | 1573-4838 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Springer |
| record_format | Article |
| series | Journal of Materials Science: Materials in Medicine |
| spelling | doaj-art-b19d4f9665854e548c49b8a60ee5c8622025-08-20T02:56:20ZengSpringerJournal of Materials Science: Materials in Medicine1573-48382025-03-0136111710.1007/s10856-025-06874-7Self-assembled injectable Icariin@ Ti3C2Tx/doxorubicin hydrogel preserving osteogenesis while synergizing photodynamic and chemodynamic therapy for osteosarcomaJiahao Fu0Hao Chen1Yiqiao Zhao2Hongzhong Xi3Yixuan Huang4Chenglin Liu5Yaokun Wu6Wei Song7Xin Liu8Bin Du9Guangquan Sun10Affiliated Hospital of Nanjing University of Chinese MedicineAffiliated Hospital of Nanjing University of Chinese MedicineSchool of Chemistry and Chemical Engineering, Nanjing University of Science and TechnologyAffiliated Hospital of Nanjing University of Chinese MedicineAffiliated Hospital of Nanjing University of Chinese MedicineAffiliated Hospital of Nanjing University of Chinese MedicineAffiliated Hospital of Nanjing University of Chinese MedicineAffiliated Hospital of Nanjing University of Chinese MedicineAffiliated Hospital of Nanjing University of Chinese MedicineAffiliated Hospital of Nanjing University of Chinese MedicineAffiliated Hospital of Nanjing University of Chinese MedicineAbstract Local therapy involving injectable hydrogel systems loaded with doxorubicin (DOX) has garnered significant attention in the realm of osteosarcoma (OS) research. Nevertheless, it has been noted that the local delivery of high-dose DOX exerts a pronounced inhibitory impact on osteogenesis, which is detrimental to the restoration of functional capabilities after OS treatment. To address this challenge, we have designed a self-assembled injectable hydrogel system that integrates photodynamic and chemodynamic therapy, aiming to enhance efficacy while mitigating adverse effects on osteogenic differentiation. In this study, an injectable sodium alginate (SA) hydrogel was fabricated by encapsulating titanium carbide powder (Ti3C2Tx) and osteoprotegerin Icariin (ICA) along with DOX. This hydrogel system demonstrated remarkable drug-loading capacity and sustained drug release. Furthermore, under near-infrared (NIR) irradiation, the hydrogel displayed outstanding photothermal effects, which, in conjunction with chemotherapy and phototherapy, effectively eradicated UMR-106 tumor cells in vitro. The incorporation of ICA not only enhanced the anti-tumor effect but also alleviated the adverse effects of DOX on the osteogenic differentiation inhibition of bone marrow mesenchymal stem cells (BMSCs). In vivo, findings further confirmed that injectable ITD/SA hydrogels can synergistically heighten anti-osteosarcoma effectiveness while mitigating local osteogenic toxicity. Given these benefits, this hydrogel holds extensive application prospects in the local therapy of OS. Graphical Abstract Schematic diagram of injectable NIR-responsive ITD/SA hydrogel phototherapy versus chemotherapy for OS and protection against BMSCS. (A) Illustration of the preparation process of the ITD/SA hydrogel. (B) Schematic of the therapeutic effect of ITD/SA under NIR irradiation.https://doi.org/10.1007/s10856-025-06874-7 |
| spellingShingle | Jiahao Fu Hao Chen Yiqiao Zhao Hongzhong Xi Yixuan Huang Chenglin Liu Yaokun Wu Wei Song Xin Liu Bin Du Guangquan Sun Self-assembled injectable Icariin@ Ti3C2Tx/doxorubicin hydrogel preserving osteogenesis while synergizing photodynamic and chemodynamic therapy for osteosarcoma Journal of Materials Science: Materials in Medicine |
| title | Self-assembled injectable Icariin@ Ti3C2Tx/doxorubicin hydrogel preserving osteogenesis while synergizing photodynamic and chemodynamic therapy for osteosarcoma |
| title_full | Self-assembled injectable Icariin@ Ti3C2Tx/doxorubicin hydrogel preserving osteogenesis while synergizing photodynamic and chemodynamic therapy for osteosarcoma |
| title_fullStr | Self-assembled injectable Icariin@ Ti3C2Tx/doxorubicin hydrogel preserving osteogenesis while synergizing photodynamic and chemodynamic therapy for osteosarcoma |
| title_full_unstemmed | Self-assembled injectable Icariin@ Ti3C2Tx/doxorubicin hydrogel preserving osteogenesis while synergizing photodynamic and chemodynamic therapy for osteosarcoma |
| title_short | Self-assembled injectable Icariin@ Ti3C2Tx/doxorubicin hydrogel preserving osteogenesis while synergizing photodynamic and chemodynamic therapy for osteosarcoma |
| title_sort | self assembled injectable icariin ti3c2tx doxorubicin hydrogel preserving osteogenesis while synergizing photodynamic and chemodynamic therapy for osteosarcoma |
| url | https://doi.org/10.1007/s10856-025-06874-7 |
| work_keys_str_mv | AT jiahaofu selfassembledinjectableicariinti3c2txdoxorubicinhydrogelpreservingosteogenesiswhilesynergizingphotodynamicandchemodynamictherapyforosteosarcoma AT haochen selfassembledinjectableicariinti3c2txdoxorubicinhydrogelpreservingosteogenesiswhilesynergizingphotodynamicandchemodynamictherapyforosteosarcoma AT yiqiaozhao selfassembledinjectableicariinti3c2txdoxorubicinhydrogelpreservingosteogenesiswhilesynergizingphotodynamicandchemodynamictherapyforosteosarcoma AT hongzhongxi selfassembledinjectableicariinti3c2txdoxorubicinhydrogelpreservingosteogenesiswhilesynergizingphotodynamicandchemodynamictherapyforosteosarcoma AT yixuanhuang selfassembledinjectableicariinti3c2txdoxorubicinhydrogelpreservingosteogenesiswhilesynergizingphotodynamicandchemodynamictherapyforosteosarcoma AT chenglinliu selfassembledinjectableicariinti3c2txdoxorubicinhydrogelpreservingosteogenesiswhilesynergizingphotodynamicandchemodynamictherapyforosteosarcoma AT yaokunwu selfassembledinjectableicariinti3c2txdoxorubicinhydrogelpreservingosteogenesiswhilesynergizingphotodynamicandchemodynamictherapyforosteosarcoma AT weisong selfassembledinjectableicariinti3c2txdoxorubicinhydrogelpreservingosteogenesiswhilesynergizingphotodynamicandchemodynamictherapyforosteosarcoma AT xinliu selfassembledinjectableicariinti3c2txdoxorubicinhydrogelpreservingosteogenesiswhilesynergizingphotodynamicandchemodynamictherapyforosteosarcoma AT bindu selfassembledinjectableicariinti3c2txdoxorubicinhydrogelpreservingosteogenesiswhilesynergizingphotodynamicandchemodynamictherapyforosteosarcoma AT guangquansun selfassembledinjectableicariinti3c2txdoxorubicinhydrogelpreservingosteogenesiswhilesynergizingphotodynamicandchemodynamictherapyforosteosarcoma |