Evidence supporting a catalytic pentad mechanism for the proteasome and other N-terminal nucleophile enzymes

Abstract Proteases are defined by their nucleophile but require additional residues to regulate their active sites, most often arranged as catalytic triads that control the generation and resolution of acyl-enzyme intermediates. Threonine N-terminal nucleophiles represent a diverse family of proteas...

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Bibliographic Details
Main Authors: Darlene Fung, Aida Razi, Michael Pandos, Benjamin Velez, Erignacio Fermin Perez, Lea Adams, Shaun Rawson, Richard M. Walsh, John Hanna
Format: Article
Language:English
Published: Nature Portfolio 2025-03-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-58077-x
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