A simplified two-plasmid system for orthogonal control of mammalian gene expression using light-activated CRISPR effector
Abstract Background Optogenetic systems use light-responsive proteins to control gene expression, ion channels, protein localization, and signaling with the “flip of a switch”. One such tool is the light activated CRISPR effector (LACE) system. Its ability to regulate gene expression in a tunable, r...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-07-01
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| Series: | BMC Biotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12896-025-00994-2 |
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