A simplified two-plasmid system for orthogonal control of mammalian gene expression using light-activated CRISPR effector

A simplified two-plasmid system for orthogonal control of mammalian gene expression using light-activated CRISPR effector

Abstract Background Optogenetic systems use light-responsive proteins to control gene expression, ion channels, protein localization, and signaling with the “flip of a switch”. One such tool is the light activated CRISPR effector (LACE) system. Its ability to regulate gene expression in a tunable, r...

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Bibliographic Details
Main Authors: Shruthi S. Garimella, Shiaki A. Minami, Anusha N. Khanchandani, Justin C. Abad Santos, Susannah R. Schaffer, Priya S. Shah
Format: Article
Language:English
Published: BMC 2025-07-01
Series:BMC Biotechnology
Subjects:
Optogenetics
CRISPR
CRY2-CIBN
Embryonic kidney cells
Myoblasts
Flow cytometry
Online Access:https://doi.org/10.1186/s12896-025-00994-2
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https://doi.org/10.1186/s12896-025-00994-2

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