Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo
We established a volumetric trans-scale imaging system with an ultra-large field-of-view (FOV) that enables simultaneous observation of millions of cellular dynamics in centimeter-wide three-dimensional (3D) tissues and embryos. Using a custom-made giant lens system with a magnification of ×2 and a...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2025-02-01
|
| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/93633 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832542845084368896 |
|---|---|
| author | Taro Ichimura Taishi Kakizuka Yoshitsugu Taniguchi Satoshi Ejima Yuki Sato Keiko Itano Kaoru Seiriki Hitoshi Hashimoto Ko Sugawara Hiroya Itoga Shuichi Onami Takeharu Nagai |
| author_facet | Taro Ichimura Taishi Kakizuka Yoshitsugu Taniguchi Satoshi Ejima Yuki Sato Keiko Itano Kaoru Seiriki Hitoshi Hashimoto Ko Sugawara Hiroya Itoga Shuichi Onami Takeharu Nagai |
| author_sort | Taro Ichimura |
| collection | DOAJ |
| description | We established a volumetric trans-scale imaging system with an ultra-large field-of-view (FOV) that enables simultaneous observation of millions of cellular dynamics in centimeter-wide three-dimensional (3D) tissues and embryos. Using a custom-made giant lens system with a magnification of ×2 and a numerical aperture (NA) of 0.25, and a CMOS camera with more than 100 megapixels, we built a trans-scale scope AMATERAS-2, and realized fluorescence imaging with a transverse spatial resolution of approximately 1.1 µm across an FOV of approximately 1.5×1.0 cm2. The 3D resolving capability was realized through a combination of optical and computational sectioning techniques tailored for our low-power imaging system. We applied the imaging technique to 1.2 cm-wide section of mouse brain, and successfully observed various regions of the brain with sub-cellular resolution in a single FOV. We also performed time-lapse imaging of a 1-cm-wide vascular network during quail embryo development for over 24 hr, visualizing the movement of over 4.0×105 vascular endothelial cells and quantitatively analyzing their dynamics. Our results demonstrate the potential of this technique in accelerating production of comprehensive reference maps of all cells in organisms and tissues, which contributes to understanding developmental processes, brain functions, and pathogenesis of disease, as well as high-throughput quality check of tissues used for transplantation medicine. |
| format | Article |
| id | doaj-art-b0deaa09adcc4bb095986c2c9bd6978d |
| institution | Kabale University |
| issn | 2050-084X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj-art-b0deaa09adcc4bb095986c2c9bd6978d2025-02-03T15:48:13ZengeLife Sciences Publications LtdeLife2050-084X2025-02-011310.7554/eLife.93633Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryoTaro Ichimura0https://orcid.org/0000-0002-3740-3634Taishi Kakizuka1Yoshitsugu Taniguchi2Satoshi Ejima3Yuki Sato4Keiko Itano5Kaoru Seiriki6Hitoshi Hashimoto7https://orcid.org/0000-0001-6548-4016Ko Sugawara8Hiroya Itoga9Shuichi Onami10https://orcid.org/0000-0002-8255-1724Takeharu Nagai11Transdimensional Life Imaging Division, Institute for Open and Transdisciplinary Research, Initiatives, Osaka University, Osaka University, Osaka, JapanTransdimensional Life Imaging Division, Institute for Open and Transdisciplinary Research, Initiatives, Osaka University, Osaka University, Osaka, Japan; Department of Biomolecular Science and Engineering, SANKEN, Osaka University, Osaka, JapanSIGMAKOKI CO LTD, Midori, Sumida-ku, Tokyo, JapanSIGMAKOKI CO LTD, Midori, Sumida-ku, Tokyo, JapanDepartment of Anatomy and Cell Biology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, JapanDepartment of Biomolecular Science and Engineering, SANKEN, Osaka University, Osaka, JapanLaboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, JapanTransdimensional Life Imaging Division, Institute for Open and Transdisciplinary Research, Initiatives, Osaka University, Osaka University, Osaka, Japan; Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, JapanLaboratory for Developmental Dynamics, RIKEN Center for Biosystems Dynamics Research, Kobe, JapanLaboratory for Developmental Dynamics, RIKEN Center for Biosystems Dynamics Research, Kobe, JapanTransdimensional Life Imaging Division, Institute for Open and Transdisciplinary Research, Initiatives, Osaka University, Osaka University, Osaka, Japan; Laboratory for Developmental Dynamics, RIKEN Center for Biosystems Dynamics Research, Kobe, JapanTransdimensional Life Imaging Division, Institute for Open and Transdisciplinary Research, Initiatives, Osaka University, Osaka University, Osaka, Japan; Department of Biomolecular Science and Engineering, SANKEN, Osaka University, Osaka, Japan; Research Institute for Electronic Science, Hokkaido University, Sapporo, JapanWe established a volumetric trans-scale imaging system with an ultra-large field-of-view (FOV) that enables simultaneous observation of millions of cellular dynamics in centimeter-wide three-dimensional (3D) tissues and embryos. Using a custom-made giant lens system with a magnification of ×2 and a numerical aperture (NA) of 0.25, and a CMOS camera with more than 100 megapixels, we built a trans-scale scope AMATERAS-2, and realized fluorescence imaging with a transverse spatial resolution of approximately 1.1 µm across an FOV of approximately 1.5×1.0 cm2. The 3D resolving capability was realized through a combination of optical and computational sectioning techniques tailored for our low-power imaging system. We applied the imaging technique to 1.2 cm-wide section of mouse brain, and successfully observed various regions of the brain with sub-cellular resolution in a single FOV. We also performed time-lapse imaging of a 1-cm-wide vascular network during quail embryo development for over 24 hr, visualizing the movement of over 4.0×105 vascular endothelial cells and quantitatively analyzing their dynamics. Our results demonstrate the potential of this technique in accelerating production of comprehensive reference maps of all cells in organisms and tissues, which contributes to understanding developmental processes, brain functions, and pathogenesis of disease, as well as high-throughput quality check of tissues used for transplantation medicine.https://elifesciences.org/articles/93633quailoptical microscopytrans-scale imaging |
| spellingShingle | Taro Ichimura Taishi Kakizuka Yoshitsugu Taniguchi Satoshi Ejima Yuki Sato Keiko Itano Kaoru Seiriki Hitoshi Hashimoto Ko Sugawara Hiroya Itoga Shuichi Onami Takeharu Nagai Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo eLife quail optical microscopy trans-scale imaging |
| title | Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo |
| title_full | Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo |
| title_fullStr | Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo |
| title_full_unstemmed | Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo |
| title_short | Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo |
| title_sort | volumetric trans scale imaging of massive quantity of heterogeneous cell populations in centimeter wide tissue and embryo |
| topic | quail optical microscopy trans-scale imaging |
| url | https://elifesciences.org/articles/93633 |
| work_keys_str_mv | AT taroichimura volumetrictransscaleimagingofmassivequantityofheterogeneouscellpopulationsincentimeterwidetissueandembryo AT taishikakizuka volumetrictransscaleimagingofmassivequantityofheterogeneouscellpopulationsincentimeterwidetissueandembryo AT yoshitsugutaniguchi volumetrictransscaleimagingofmassivequantityofheterogeneouscellpopulationsincentimeterwidetissueandembryo AT satoshiejima volumetrictransscaleimagingofmassivequantityofheterogeneouscellpopulationsincentimeterwidetissueandembryo AT yukisato volumetrictransscaleimagingofmassivequantityofheterogeneouscellpopulationsincentimeterwidetissueandembryo AT keikoitano volumetrictransscaleimagingofmassivequantityofheterogeneouscellpopulationsincentimeterwidetissueandembryo AT kaoruseiriki volumetrictransscaleimagingofmassivequantityofheterogeneouscellpopulationsincentimeterwidetissueandembryo AT hitoshihashimoto volumetrictransscaleimagingofmassivequantityofheterogeneouscellpopulationsincentimeterwidetissueandembryo AT kosugawara volumetrictransscaleimagingofmassivequantityofheterogeneouscellpopulationsincentimeterwidetissueandembryo AT hiroyaitoga volumetrictransscaleimagingofmassivequantityofheterogeneouscellpopulationsincentimeterwidetissueandembryo AT shuichionami volumetrictransscaleimagingofmassivequantityofheterogeneouscellpopulationsincentimeterwidetissueandembryo AT takeharunagai volumetrictransscaleimagingofmassivequantityofheterogeneouscellpopulationsincentimeterwidetissueandembryo |