Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo

We established a volumetric trans-scale imaging system with an ultra-large field-of-view (FOV) that enables simultaneous observation of millions of cellular dynamics in centimeter-wide three-dimensional (3D) tissues and embryos. Using a custom-made giant lens system with a magnification of ×2 and a...

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Main Authors: Taro Ichimura, Taishi Kakizuka, Yoshitsugu Taniguchi, Satoshi Ejima, Yuki Sato, Keiko Itano, Kaoru Seiriki, Hitoshi Hashimoto, Ko Sugawara, Hiroya Itoga, Shuichi Onami, Takeharu Nagai
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Language:English
Published: eLife Sciences Publications Ltd 2025-02-01
Series:eLife
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Online Access:https://elifesciences.org/articles/93633
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author Taro Ichimura
Taishi Kakizuka
Yoshitsugu Taniguchi
Satoshi Ejima
Yuki Sato
Keiko Itano
Kaoru Seiriki
Hitoshi Hashimoto
Ko Sugawara
Hiroya Itoga
Shuichi Onami
Takeharu Nagai
author_facet Taro Ichimura
Taishi Kakizuka
Yoshitsugu Taniguchi
Satoshi Ejima
Yuki Sato
Keiko Itano
Kaoru Seiriki
Hitoshi Hashimoto
Ko Sugawara
Hiroya Itoga
Shuichi Onami
Takeharu Nagai
author_sort Taro Ichimura
collection DOAJ
description We established a volumetric trans-scale imaging system with an ultra-large field-of-view (FOV) that enables simultaneous observation of millions of cellular dynamics in centimeter-wide three-dimensional (3D) tissues and embryos. Using a custom-made giant lens system with a magnification of ×2 and a numerical aperture (NA) of 0.25, and a CMOS camera with more than 100 megapixels, we built a trans-scale scope AMATERAS-2, and realized fluorescence imaging with a transverse spatial resolution of approximately 1.1 µm across an FOV of approximately 1.5×1.0 cm2. The 3D resolving capability was realized through a combination of optical and computational sectioning techniques tailored for our low-power imaging system. We applied the imaging technique to 1.2 cm-wide section of mouse brain, and successfully observed various regions of the brain with sub-cellular resolution in a single FOV. We also performed time-lapse imaging of a 1-cm-wide vascular network during quail embryo development for over 24 hr, visualizing the movement of over 4.0×105 vascular endothelial cells and quantitatively analyzing their dynamics. Our results demonstrate the potential of this technique in accelerating production of comprehensive reference maps of all cells in organisms and tissues, which contributes to understanding developmental processes, brain functions, and pathogenesis of disease, as well as high-throughput quality check of tissues used for transplantation medicine.
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spelling doaj-art-b0deaa09adcc4bb095986c2c9bd6978d2025-02-03T15:48:13ZengeLife Sciences Publications LtdeLife2050-084X2025-02-011310.7554/eLife.93633Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryoTaro Ichimura0https://orcid.org/0000-0002-3740-3634Taishi Kakizuka1Yoshitsugu Taniguchi2Satoshi Ejima3Yuki Sato4Keiko Itano5Kaoru Seiriki6Hitoshi Hashimoto7https://orcid.org/0000-0001-6548-4016Ko Sugawara8Hiroya Itoga9Shuichi Onami10https://orcid.org/0000-0002-8255-1724Takeharu Nagai11Transdimensional Life Imaging Division, Institute for Open and Transdisciplinary Research, Initiatives, Osaka University, Osaka University, Osaka, JapanTransdimensional Life Imaging Division, Institute for Open and Transdisciplinary Research, Initiatives, Osaka University, Osaka University, Osaka, Japan; Department of Biomolecular Science and Engineering, SANKEN, Osaka University, Osaka, JapanSIGMAKOKI CO LTD, Midori, Sumida-ku, Tokyo, JapanSIGMAKOKI CO LTD, Midori, Sumida-ku, Tokyo, JapanDepartment of Anatomy and Cell Biology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, JapanDepartment of Biomolecular Science and Engineering, SANKEN, Osaka University, Osaka, JapanLaboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, JapanTransdimensional Life Imaging Division, Institute for Open and Transdisciplinary Research, Initiatives, Osaka University, Osaka University, Osaka, Japan; Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, JapanLaboratory for Developmental Dynamics, RIKEN Center for Biosystems Dynamics Research, Kobe, JapanLaboratory for Developmental Dynamics, RIKEN Center for Biosystems Dynamics Research, Kobe, JapanTransdimensional Life Imaging Division, Institute for Open and Transdisciplinary Research, Initiatives, Osaka University, Osaka University, Osaka, Japan; Laboratory for Developmental Dynamics, RIKEN Center for Biosystems Dynamics Research, Kobe, JapanTransdimensional Life Imaging Division, Institute for Open and Transdisciplinary Research, Initiatives, Osaka University, Osaka University, Osaka, Japan; Department of Biomolecular Science and Engineering, SANKEN, Osaka University, Osaka, Japan; Research Institute for Electronic Science, Hokkaido University, Sapporo, JapanWe established a volumetric trans-scale imaging system with an ultra-large field-of-view (FOV) that enables simultaneous observation of millions of cellular dynamics in centimeter-wide three-dimensional (3D) tissues and embryos. Using a custom-made giant lens system with a magnification of ×2 and a numerical aperture (NA) of 0.25, and a CMOS camera with more than 100 megapixels, we built a trans-scale scope AMATERAS-2, and realized fluorescence imaging with a transverse spatial resolution of approximately 1.1 µm across an FOV of approximately 1.5×1.0 cm2. The 3D resolving capability was realized through a combination of optical and computational sectioning techniques tailored for our low-power imaging system. We applied the imaging technique to 1.2 cm-wide section of mouse brain, and successfully observed various regions of the brain with sub-cellular resolution in a single FOV. We also performed time-lapse imaging of a 1-cm-wide vascular network during quail embryo development for over 24 hr, visualizing the movement of over 4.0×105 vascular endothelial cells and quantitatively analyzing their dynamics. Our results demonstrate the potential of this technique in accelerating production of comprehensive reference maps of all cells in organisms and tissues, which contributes to understanding developmental processes, brain functions, and pathogenesis of disease, as well as high-throughput quality check of tissues used for transplantation medicine.https://elifesciences.org/articles/93633quailoptical microscopytrans-scale imaging
spellingShingle Taro Ichimura
Taishi Kakizuka
Yoshitsugu Taniguchi
Satoshi Ejima
Yuki Sato
Keiko Itano
Kaoru Seiriki
Hitoshi Hashimoto
Ko Sugawara
Hiroya Itoga
Shuichi Onami
Takeharu Nagai
Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo
eLife
quail
optical microscopy
trans-scale imaging
title Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo
title_full Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo
title_fullStr Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo
title_full_unstemmed Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo
title_short Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo
title_sort volumetric trans scale imaging of massive quantity of heterogeneous cell populations in centimeter wide tissue and embryo
topic quail
optical microscopy
trans-scale imaging
url https://elifesciences.org/articles/93633
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