Quantitative Detection of Low-Copy-Number mRNAs Differing at Single Nucleotide Positions
Accurate analysis of mRNA expression levels of SNPs, highly homologous genes, and splicing variants requires techniques capable of quantifying low-copy-number mRNAs differing at single nucleotide positions. We have used an RT-PCR-based technique based on co-amplification of closely related target mR...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2003-01-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/03341dd05 |
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