Two-color fluorescent in situ hybridization using chromogenic substrates in zebrafish

Two-color fluorescent in situ hybridization (FISH) is a widely used technique for comparing relative gene expression patterns. Current two-color FISH protocols are not ideal for detecting weakly expressed transcripts or monitoring signal strength and background levels during the course of the reacti...

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Bibliographic Details
Main Authors: Jennifer A. Schumacher, Emma J. Zhao, Matthew J. Kofron, Saulius Sumanas
Format: Article
Language:English
Published: Taylor & Francis Group 2014-11-01
Series:BioTechniques
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Online Access:https://www.future-science.com/doi/10.2144/000114229
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