A Comparative Study on Phenotypic versus ITS-Based Molecular Identification of Dermatophytes Isolated in Dakar, Senegal
Classically, dermatophytes are identified by phenotypic methods even if these methods, sometimes, remain difficult or uncertain. On the other hand, nucleotide sequence analysis of internal transcribed spacers (ITS) of rDNA has proved to be a useful method for identification of dermatophytes. The obj...
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| Format: | Article |
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Wiley
2019-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2019/6754058 |
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| author | Khadim Diongue Ludivine Bréchard Mamadou Alpha Diallo Mame Cheikh Seck Mouhamadou Ndiaye Aida Sadikh Badiane Stéphane Ranque Daouda Ndiaye |
| author_facet | Khadim Diongue Ludivine Bréchard Mamadou Alpha Diallo Mame Cheikh Seck Mouhamadou Ndiaye Aida Sadikh Badiane Stéphane Ranque Daouda Ndiaye |
| author_sort | Khadim Diongue |
| collection | DOAJ |
| description | Classically, dermatophytes are identified by phenotypic methods even if these methods, sometimes, remain difficult or uncertain. On the other hand, nucleotide sequence analysis of internal transcribed spacers (ITS) of rDNA has proved to be a useful method for identification of dermatophytes. The objective of this study was to compare the phenotypic method with DNA sequencing of the ITS regions for identification of dermatophyte species isolated in Dakar, Senegal. A collection of thirty-two strains of dermatophytes were isolated from patients suffering from dermatophytosis. Mycological identification revealed Trichophyton soudanense (n = 13), T. interdigitale (n = 10), Microsporum audouinii (n = 5), and one strain for each of the following species: T. rubrum, T. mentagrophytes, and M. canis and one unidentified strain. For comparison, ITS-based PCR and DNA sequencing were applied for identification of the isolated dermatophytes. ITS sequences showed, in BLAST search analysis, 99-100% of similarity. Identification of dermatophyte isolates by conventional methods was confirmed by DNA sequencing of the ITS regions in 84% of cases. Discrepancies concern mostly T. rubrum misidentified as T. interdigitale. PCR sequencing provided an excellent tool for identifying dermatophyte strains that do not present typical morphological characteristics. It was also able to give correct identification of an atypical strain of M. audouinii responsible of mycetoma of the scalp. |
| format | Article |
| id | doaj-art-b038f7ff9c6d4c4aa6946f904861c221 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-b038f7ff9c6d4c4aa6946f904861c2212025-02-03T01:28:11ZengWileyInternational Journal of Microbiology1687-918X1687-91982019-01-01201910.1155/2019/67540586754058A Comparative Study on Phenotypic versus ITS-Based Molecular Identification of Dermatophytes Isolated in Dakar, SenegalKhadim Diongue0Ludivine Bréchard1Mamadou Alpha Diallo2Mame Cheikh Seck3Mouhamadou Ndiaye4Aida Sadikh Badiane5Stéphane Ranque6Daouda Ndiaye7Laboratoire de Parasitologie-Mycologie, CHU Aristide Le Dantec, BP 5005, Dakar, SenegalInstitut Hospitalo-Universitaire Méditerranée Infection, 13 005 Marseille, FranceLaboratoire de Parasitologie-Mycologie, CHU Aristide Le Dantec, BP 5005, Dakar, SenegalLaboratoire de Parasitologie-Mycologie, CHU Aristide Le Dantec, BP 5005, Dakar, SenegalLaboratoire de Parasitologie-Mycologie, CHU Aristide Le Dantec, BP 5005, Dakar, SenegalLaboratoire de Parasitologie-Mycologie, CHU Aristide Le Dantec, BP 5005, Dakar, SenegalInstitut Hospitalo-Universitaire Méditerranée Infection, 13 005 Marseille, FranceLaboratoire de Parasitologie-Mycologie, CHU Aristide Le Dantec, BP 5005, Dakar, SenegalClassically, dermatophytes are identified by phenotypic methods even if these methods, sometimes, remain difficult or uncertain. On the other hand, nucleotide sequence analysis of internal transcribed spacers (ITS) of rDNA has proved to be a useful method for identification of dermatophytes. The objective of this study was to compare the phenotypic method with DNA sequencing of the ITS regions for identification of dermatophyte species isolated in Dakar, Senegal. A collection of thirty-two strains of dermatophytes were isolated from patients suffering from dermatophytosis. Mycological identification revealed Trichophyton soudanense (n = 13), T. interdigitale (n = 10), Microsporum audouinii (n = 5), and one strain for each of the following species: T. rubrum, T. mentagrophytes, and M. canis and one unidentified strain. For comparison, ITS-based PCR and DNA sequencing were applied for identification of the isolated dermatophytes. ITS sequences showed, in BLAST search analysis, 99-100% of similarity. Identification of dermatophyte isolates by conventional methods was confirmed by DNA sequencing of the ITS regions in 84% of cases. Discrepancies concern mostly T. rubrum misidentified as T. interdigitale. PCR sequencing provided an excellent tool for identifying dermatophyte strains that do not present typical morphological characteristics. It was also able to give correct identification of an atypical strain of M. audouinii responsible of mycetoma of the scalp.http://dx.doi.org/10.1155/2019/6754058 |
| spellingShingle | Khadim Diongue Ludivine Bréchard Mamadou Alpha Diallo Mame Cheikh Seck Mouhamadou Ndiaye Aida Sadikh Badiane Stéphane Ranque Daouda Ndiaye A Comparative Study on Phenotypic versus ITS-Based Molecular Identification of Dermatophytes Isolated in Dakar, Senegal International Journal of Microbiology |
| title | A Comparative Study on Phenotypic versus ITS-Based Molecular Identification of Dermatophytes Isolated in Dakar, Senegal |
| title_full | A Comparative Study on Phenotypic versus ITS-Based Molecular Identification of Dermatophytes Isolated in Dakar, Senegal |
| title_fullStr | A Comparative Study on Phenotypic versus ITS-Based Molecular Identification of Dermatophytes Isolated in Dakar, Senegal |
| title_full_unstemmed | A Comparative Study on Phenotypic versus ITS-Based Molecular Identification of Dermatophytes Isolated in Dakar, Senegal |
| title_short | A Comparative Study on Phenotypic versus ITS-Based Molecular Identification of Dermatophytes Isolated in Dakar, Senegal |
| title_sort | comparative study on phenotypic versus its based molecular identification of dermatophytes isolated in dakar senegal |
| url | http://dx.doi.org/10.1155/2019/6754058 |
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