Immobilization of DNA Aptamers on Polyester Cloth for Antigen Detection by Dot Blot Immunoenzymatic Assay (Aptablot)

A simple dot blot immunoenzymatic assay system was developed using polyester cloth coated with an oligo-DNA aptamer to provide a high-affinity macroporous surface for the efficient capture of a model protein analyte (thrombin) in complex sample matrices such as foods. Bound thrombin was detected imm...

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Bibliographic Details
Main Authors: Sally Smiley, Maria DeRosa, Burton Blais
Format: Article
Language:English
Published: Wiley 2013-01-01
Series:Journal of Nucleic Acids
Online Access:http://dx.doi.org/10.1155/2013/936542
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