Prokaryotic expression of the coat protein gene of Odontoglossum ringspot virus, preparation and application of its polyclonal antibodies

Prokaryotic expression of the coat protein gene of Odontoglossum ringspot virus, preparation and application of its polyclonal antibodies

Odontoglossum ringspot virus (ORSV) is one of the most prevalent orchid viruses. The 477 bp coat protein gene (CP) of ORSV was cloned from the virus infected orchid samples using RT-PCR, and subcloned into a prokaryotic expression vector pET-32a. The recombinant prokaryotic expression vector (pET-32...

Full description

Saved in:
Bibliographic Details
Main Authors: ZHANG Jie, MENG Chun-mei, RONG Song, ZHANG Chao, HONG Jian, WU Jian-xiang
Format: Article
Language:English
Published: Zhejiang University Press 2010-07-01
Series:浙江大学学报. 农业与生命科学版
Subjects:
<italic>Odontoglossum ringspot virus</italic>
prokaryotic expression
polyclonal antibody
dot-blot ELISA
immunocapture RT-PCR
Online Access:https://www.academax.com/doi/10.3785/j.issn.1008-9209.2010.04.004
Tags: Add Tag
No Tags, Be the first to tag this record!

Internet

https://www.academax.com/doi/10.3785/j.issn.1008-9209.2010.04.004

Similar Items