Resolving acetylated and phosphorylated proteins by neutral urea Triton-polyacrylamide gel electrophoresis: NUT-PAGE

Protein acetylation and phosphorylation are key modifications that regulate both normal and pathological protein functions. The gel systems currently used for analyzing modified proteins require either expensive reagents or time-consuming second dimension electrophoresis. Here we present a neutral p...

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Bibliographic Details
Main Authors: Christopher J. Buehl, Xiexiong Deng, Mengyu Liu, Michael J McAndrew, Stacy Hovde, Xinjing Xu, Min-Hao Kuo
Format: Article
Language:English
Published: Taylor & Francis Group 2014-08-01
Series:BioTechniques
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Online Access:https://www.future-science.com/doi/10.2144/000114197
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