Methods for preliminary determination of pemetrexed in macromolecular drug-carrier systems
Pemetrexed (PMX) is an antifolate drug utilized in the treatment of non-small cell lung cancer. For studies of potential macromolecular carriers for PMX, fast and precise methods were developed to determine the bound and free drug contained in investigated conjugate preparations. The analysis of the...
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| Format: | Article |
| Language: | English |
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Sciendo
2016-03-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.1515/acph-2016-0001 |
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| author | Ciekot Jarosław Goszczyński Tomasz Marek Boratyński Janusz |
| author_facet | Ciekot Jarosław Goszczyński Tomasz Marek Boratyński Janusz |
| author_sort | Ciekot Jarosław |
| collection | DOAJ |
| description | Pemetrexed (PMX) is an antifolate drug utilized in the treatment of non-small cell lung cancer. For studies of potential macromolecular carriers for PMX, fast and precise methods were developed to determine the bound and free drug contained in investigated conjugate preparations. The analysis of the total amount of PMX in conjugates was based on absorption spectrophotometry. The linearity was found in the range of 4.697–46.97 μmol L−1 PMX. The limit of quantitation was 1.070 μmol L−1. The method for the analysis of unbound PMX was based on size-exclusion chromatography and detection at 225 nm. This method shows linear range of 2.230–223.0 μmol L−1. LOQ was 0.539 μmol L−1. The proposed methods can be used both for the characterization of the polysaccharide based conjugates of PMX and for the determination of conjugate drug release profiles. |
| format | Article |
| id | doaj-art-ad61c6f5b2cc4850b3119f3324fc18e7 |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2016-03-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-ad61c6f5b2cc4850b3119f3324fc18e72025-02-02T09:44:14ZengSciendoActa Pharmaceutica1846-95582016-03-0166114715310.1515/acph-2016-0001acph-2016-0001Methods for preliminary determination of pemetrexed in macromolecular drug-carrier systemsCiekot Jarosław0Goszczyński Tomasz Marek1Boratyński Janusz2 ”NeoLek” Laboratory, Department of Experimental Oncology, Ludwik, Hirszfeld Institute of Immunology and Experimental Therapy, PAS, Wrocław 53-114, Poland ”NeoLek” Laboratory, Department of Experimental Oncology, Ludwik, Hirszfeld Institute of Immunology and Experimental Therapy, PAS, Wrocław 53-114, Poland ”NeoLek” Laboratory, Department of Experimental Oncology, Ludwik, Hirszfeld Institute of Immunology and Experimental Therapy, PAS, Wrocław 53-114, PolandPemetrexed (PMX) is an antifolate drug utilized in the treatment of non-small cell lung cancer. For studies of potential macromolecular carriers for PMX, fast and precise methods were developed to determine the bound and free drug contained in investigated conjugate preparations. The analysis of the total amount of PMX in conjugates was based on absorption spectrophotometry. The linearity was found in the range of 4.697–46.97 μmol L−1 PMX. The limit of quantitation was 1.070 μmol L−1. The method for the analysis of unbound PMX was based on size-exclusion chromatography and detection at 225 nm. This method shows linear range of 2.230–223.0 μmol L−1. LOQ was 0.539 μmol L−1. The proposed methods can be used both for the characterization of the polysaccharide based conjugates of PMX and for the determination of conjugate drug release profiles.https://doi.org/10.1515/acph-2016-0001pemetrexedmacromolecular drug-carriersize-exclusion chromatographyultraviolet spectroscopy |
| spellingShingle | Ciekot Jarosław Goszczyński Tomasz Marek Boratyński Janusz Methods for preliminary determination of pemetrexed in macromolecular drug-carrier systems Acta Pharmaceutica pemetrexed macromolecular drug-carrier size-exclusion chromatography ultraviolet spectroscopy |
| title | Methods for preliminary determination of pemetrexed in macromolecular drug-carrier systems |
| title_full | Methods for preliminary determination of pemetrexed in macromolecular drug-carrier systems |
| title_fullStr | Methods for preliminary determination of pemetrexed in macromolecular drug-carrier systems |
| title_full_unstemmed | Methods for preliminary determination of pemetrexed in macromolecular drug-carrier systems |
| title_short | Methods for preliminary determination of pemetrexed in macromolecular drug-carrier systems |
| title_sort | methods for preliminary determination of pemetrexed in macromolecular drug carrier systems |
| topic | pemetrexed macromolecular drug-carrier size-exclusion chromatography ultraviolet spectroscopy |
| url | https://doi.org/10.1515/acph-2016-0001 |
| work_keys_str_mv | AT ciekotjarosław methodsforpreliminarydeterminationofpemetrexedinmacromoleculardrugcarriersystems AT goszczynskitomaszmarek methodsforpreliminarydeterminationofpemetrexedinmacromoleculardrugcarriersystems AT boratynskijanusz methodsforpreliminarydeterminationofpemetrexedinmacromoleculardrugcarriersystems |