Assessment of Global and Local Alterations in Retinal Layer Thickness in Ins2 (Akita) Diabetic Mice by Spectral Domain Optical Coherence Tomography
Purpose/Aim. The Ins2 (Akita) mouse is a spontaneous diabetic mouse model with a heterozygous mutation in the insulin 2 gene that results in sustained hyperglycemia. The purpose of the study was to assess global and local retinal layer thickness alterations in Akita mice by analysis of spectral doma...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2018/7253498 |
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| author | Andrew W. Francis Justin Wanek Mahnaz Shahidi |
| author_facet | Andrew W. Francis Justin Wanek Mahnaz Shahidi |
| author_sort | Andrew W. Francis |
| collection | DOAJ |
| description | Purpose/Aim. The Ins2 (Akita) mouse is a spontaneous diabetic mouse model with a heterozygous mutation in the insulin 2 gene that results in sustained hyperglycemia. The purpose of the study was to assess global and local retinal layer thickness alterations in Akita mice by analysis of spectral domain optical coherence tomography (SD-OCT) images. Materials and Methods. SD-OCT imaging was performed in Akita and wild-type mice at 12 and 24 weeks of age. Inner retinal thickness (IRT), outer retinal thickness (ORT), total retinal thickness (TRT), and photoreceptor outer segment length (OSL) were measured. Mean global thickness values were compared between Akita and wild-type mice. Local thickness variations in Akita mice were assessed based on normative values in wild-type mice. Results. Akita mice had higher blood glucose levels and lower body weights (p<0.001). On average, IRT, ORT, and TRT were approximately 2% lower in Akita mice than in wild-type mice (p≤0.02). In Akita mice, the percent difference between retinal areas with thickness below and above normative values for IRT, ORT, and TRT was 22%, 32%, and 38%, respectively. Conclusions. These findings support the use of the Akita mouse model to study the retinal neurodegenerative effects of hyperglycemia. |
| format | Article |
| id | doaj-art-ad3e1eb0ddd24dd2aaba3e9bc2c25da9 |
| institution | Kabale University |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-ad3e1eb0ddd24dd2aaba3e9bc2c25da92025-02-03T06:12:02ZengWileyJournal of Ophthalmology2090-004X2090-00582018-01-01201810.1155/2018/72534987253498Assessment of Global and Local Alterations in Retinal Layer Thickness in Ins2 (Akita) Diabetic Mice by Spectral Domain Optical Coherence TomographyAndrew W. Francis0Justin Wanek1Mahnaz Shahidi2Department of Ophthalmology, University of California San Francisco, San Francisco, CA, USADepartment of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL, USADepartment of Ophthalmology, University of Southern California, Los Angeles, CA, USAPurpose/Aim. The Ins2 (Akita) mouse is a spontaneous diabetic mouse model with a heterozygous mutation in the insulin 2 gene that results in sustained hyperglycemia. The purpose of the study was to assess global and local retinal layer thickness alterations in Akita mice by analysis of spectral domain optical coherence tomography (SD-OCT) images. Materials and Methods. SD-OCT imaging was performed in Akita and wild-type mice at 12 and 24 weeks of age. Inner retinal thickness (IRT), outer retinal thickness (ORT), total retinal thickness (TRT), and photoreceptor outer segment length (OSL) were measured. Mean global thickness values were compared between Akita and wild-type mice. Local thickness variations in Akita mice were assessed based on normative values in wild-type mice. Results. Akita mice had higher blood glucose levels and lower body weights (p<0.001). On average, IRT, ORT, and TRT were approximately 2% lower in Akita mice than in wild-type mice (p≤0.02). In Akita mice, the percent difference between retinal areas with thickness below and above normative values for IRT, ORT, and TRT was 22%, 32%, and 38%, respectively. Conclusions. These findings support the use of the Akita mouse model to study the retinal neurodegenerative effects of hyperglycemia.http://dx.doi.org/10.1155/2018/7253498 |
| spellingShingle | Andrew W. Francis Justin Wanek Mahnaz Shahidi Assessment of Global and Local Alterations in Retinal Layer Thickness in Ins2 (Akita) Diabetic Mice by Spectral Domain Optical Coherence Tomography Journal of Ophthalmology |
| title | Assessment of Global and Local Alterations in Retinal Layer Thickness in Ins2 (Akita) Diabetic Mice by Spectral Domain Optical Coherence Tomography |
| title_full | Assessment of Global and Local Alterations in Retinal Layer Thickness in Ins2 (Akita) Diabetic Mice by Spectral Domain Optical Coherence Tomography |
| title_fullStr | Assessment of Global and Local Alterations in Retinal Layer Thickness in Ins2 (Akita) Diabetic Mice by Spectral Domain Optical Coherence Tomography |
| title_full_unstemmed | Assessment of Global and Local Alterations in Retinal Layer Thickness in Ins2 (Akita) Diabetic Mice by Spectral Domain Optical Coherence Tomography |
| title_short | Assessment of Global and Local Alterations in Retinal Layer Thickness in Ins2 (Akita) Diabetic Mice by Spectral Domain Optical Coherence Tomography |
| title_sort | assessment of global and local alterations in retinal layer thickness in ins2 akita diabetic mice by spectral domain optical coherence tomography |
| url | http://dx.doi.org/10.1155/2018/7253498 |
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