Is There a Key Primer for Amplification of Core Land Plant DNA Barcode Regions (rbcL and matK)?

Is There a Key Primer for Amplification of Core Land Plant DNA Barcode Regions (rbcL and matK)?

ABSTRACT The DNA barcode is a technique for molecular identification of species. Two core genes, matK and rbcL, are widely used for land plants. In this technique, the selection of primers is a fundamental step for the success of amplification. Then, we aim to evaluate the primer amplification capab...

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Bibliographic Details
Main Authors: Leonardo C. J. Corvalán, Amanda A. deMelo‐Ximenes, Larissa R. Carvalho, Carlos de M. e Silva‐Neto, José A. F. Diniz‐Filho, Mariana P. de C. Telles, Rhewter Nunes
Format: Article
Language:English
Published: Wiley 2025-02-01
Series:Ecology and Evolution
Subjects:
annealing evaluation
DNA metabarcode
in silico PCR
molecular identification
primer coverage
Online Access:https://doi.org/10.1002/ece3.70961
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https://doi.org/10.1002/ece3.70961

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