Optimization of TaqMan-based quantitative PCR diagnosis for Entamoeba histolytica using droplet digital PCR.

Optimization of TaqMan-based quantitative PCR diagnosis for Entamoeba histolytica using droplet digital PCR.

<h4>Background</h4>TaqMan-probed quantitative PCR (qPCR) is highly valued for diagnosing Entamoeba histolytica infections (amebiasis). However, unclear cycle threshold (Ct) values often yield low-titer positive results, complicating interpretation. This study aimed to optimize qPCR prime...

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Bibliographic Details
Main Authors: Akira Kawashima, Yasuaki Yanagawa, Takayuki Chikata, Rieko Shimogawara, Daisuke Mizushima, Kiyoto Tsuchiya, Kenji Yagita, Hiroyuki Gatanaga, Koji Watanabe
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2025-06-01
Series:PLoS Neglected Tropical Diseases
Online Access:https://doi.org/10.1371/journal.pntd.0012935
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https://doi.org/10.1371/journal.pntd.0012935

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