Full-Length cDNA Cloning and Determination of mRNA 5′and 3′Ends by Amplification of Adaptor-Ligated cDNA
An efficient cDNA amplification procedure is described for determining of the 5′ and 3′ ends of mRNAs and cloning full-length cDNAs. In this approach, a double-stranded (ds) adaptor is ligated to both ends of a library of ds cDNA by T4 DNA ligase. This adaptor-ligated ds cDNA is then used to selecti...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1996-09-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/96213pf02 |
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