Transcriptome Sequencing Revealed an Inhibitory Mechanism of Recombinant Puroindoline B Protein on <i>Aspergillus flavus</i>
<i>Aspergillus flavus</i>, a common food contaminant, poses health and economic risks. Previous research showed that recombinant Puroindoline B protein (rPINB) inhibited <i>A. flavus</i> by disrupting its cell wall, membrane, nuclear function, mitochondrial activity, and oxid...
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2025-05-01
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| author | Pingping Tian Cuixiang Li Yangyong Lv Shaobin Gu Yuansen Hu |
| author_facet | Pingping Tian Cuixiang Li Yangyong Lv Shaobin Gu Yuansen Hu |
| author_sort | Pingping Tian |
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| description | <i>Aspergillus flavus</i>, a common food contaminant, poses health and economic risks. Previous research showed that recombinant Puroindoline B protein (rPINB) inhibited <i>A. flavus</i> by disrupting its cell wall, membrane, nuclear function, mitochondrial activity, and oxidative stress. This study used transcriptome technology to explore the impact of rPINB on <i>A. flavus</i> gene expression and created gene deletion strains to test the sensitivity to rPINB. RNA-Seq identified the differentially expressed genes (DEGs) affecting cell wall synthesis, membrane transport, oxidative stress, spore formation, and aflatoxin production. The MFS transporter genes AFLA_106900 (<i>mfs1</i>) and AFLA_106910 (<i>mfs2</i>) were crucial for an inhibitory effect of rPINB. The mutants exhibited reduced sensitivity to rPINB-mediated inhibition, indicating lower growth, sunken conidia, and shriveled hyphae, compared to the wild-type strain. The results also demonstrated decreased sensitivity to the stress agents affecting cell membranes, osmotic balance, and oxidation, alongside a significant reduction in AFB1 production in gene-deleted strains. These results suggested that <i>mfs1</i> and <i>mfs2</i> were essential for rPINB protein’s inhibition of <i>A. flavus</i> growth, laying the groundwork for the mold control strategies using plant proteins. |
| format | Article |
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| language | English |
| publishDate | 2025-05-01 |
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| spelling | doaj-art-ac3f2803ce6a41268ff1a707bd96e3aa2025-08-20T02:23:05ZengMDPI AGFoods2304-81582025-05-011411190310.3390/foods14111903Transcriptome Sequencing Revealed an Inhibitory Mechanism of Recombinant Puroindoline B Protein on <i>Aspergillus flavus</i>Pingping Tian0Cuixiang Li1Yangyong Lv2Shaobin Gu3Yuansen Hu4College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, ChinaCollege of Biological Engineering, Henan University of Technology, Zhengzhou 450001, ChinaCollege of Biological Engineering, Henan University of Technology, Zhengzhou 450001, ChinaCollege of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, ChinaCollege of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China<i>Aspergillus flavus</i>, a common food contaminant, poses health and economic risks. Previous research showed that recombinant Puroindoline B protein (rPINB) inhibited <i>A. flavus</i> by disrupting its cell wall, membrane, nuclear function, mitochondrial activity, and oxidative stress. This study used transcriptome technology to explore the impact of rPINB on <i>A. flavus</i> gene expression and created gene deletion strains to test the sensitivity to rPINB. RNA-Seq identified the differentially expressed genes (DEGs) affecting cell wall synthesis, membrane transport, oxidative stress, spore formation, and aflatoxin production. The MFS transporter genes AFLA_106900 (<i>mfs1</i>) and AFLA_106910 (<i>mfs2</i>) were crucial for an inhibitory effect of rPINB. The mutants exhibited reduced sensitivity to rPINB-mediated inhibition, indicating lower growth, sunken conidia, and shriveled hyphae, compared to the wild-type strain. The results also demonstrated decreased sensitivity to the stress agents affecting cell membranes, osmotic balance, and oxidation, alongside a significant reduction in AFB1 production in gene-deleted strains. These results suggested that <i>mfs1</i> and <i>mfs2</i> were essential for rPINB protein’s inhibition of <i>A. flavus</i> growth, laying the groundwork for the mold control strategies using plant proteins.https://www.mdpi.com/2304-8158/14/11/1903Puroindoline B protein<i>Aspergillus flavus</i>RNA-seqmajor facilitator superfamily transporters |
| spellingShingle | Pingping Tian Cuixiang Li Yangyong Lv Shaobin Gu Yuansen Hu Transcriptome Sequencing Revealed an Inhibitory Mechanism of Recombinant Puroindoline B Protein on <i>Aspergillus flavus</i> Foods Puroindoline B protein <i>Aspergillus flavus</i> RNA-seq major facilitator superfamily transporters |
| title | Transcriptome Sequencing Revealed an Inhibitory Mechanism of Recombinant Puroindoline B Protein on <i>Aspergillus flavus</i> |
| title_full | Transcriptome Sequencing Revealed an Inhibitory Mechanism of Recombinant Puroindoline B Protein on <i>Aspergillus flavus</i> |
| title_fullStr | Transcriptome Sequencing Revealed an Inhibitory Mechanism of Recombinant Puroindoline B Protein on <i>Aspergillus flavus</i> |
| title_full_unstemmed | Transcriptome Sequencing Revealed an Inhibitory Mechanism of Recombinant Puroindoline B Protein on <i>Aspergillus flavus</i> |
| title_short | Transcriptome Sequencing Revealed an Inhibitory Mechanism of Recombinant Puroindoline B Protein on <i>Aspergillus flavus</i> |
| title_sort | transcriptome sequencing revealed an inhibitory mechanism of recombinant puroindoline b protein on i aspergillus flavus i |
| topic | Puroindoline B protein <i>Aspergillus flavus</i> RNA-seq major facilitator superfamily transporters |
| url | https://www.mdpi.com/2304-8158/14/11/1903 |
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