Transcriptome Sequencing Revealed an Inhibitory Mechanism of Recombinant Puroindoline B Protein on <i>Aspergillus flavus</i>

Transcriptome Sequencing Revealed an Inhibitory Mechanism of Recombinant Puroindoline B Protein on <i>Aspergillus flavus</i>

<i>Aspergillus flavus</i>, a common food contaminant, poses health and economic risks. Previous research showed that recombinant Puroindoline B protein (rPINB) inhibited <i>A. flavus</i> by disrupting its cell wall, membrane, nuclear function, mitochondrial activity, and oxid...

Full description

Saved in:
Bibliographic Details
Main Authors: Pingping Tian, Cuixiang Li, Yangyong Lv, Shaobin Gu, Yuansen Hu
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Foods
Subjects:
Puroindoline B protein
<i>Aspergillus flavus</i>
RNA-seq
major facilitator superfamily transporters
Online Access:https://www.mdpi.com/2304-8158/14/11/1903
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:<i>Aspergillus flavus</i>, a common food contaminant, poses health and economic risks. Previous research showed that recombinant Puroindoline B protein (rPINB) inhibited <i>A. flavus</i> by disrupting its cell wall, membrane, nuclear function, mitochondrial activity, and oxidative stress. This study used transcriptome technology to explore the impact of rPINB on <i>A. flavus</i> gene expression and created gene deletion strains to test the sensitivity to rPINB. RNA-Seq identified the differentially expressed genes (DEGs) affecting cell wall synthesis, membrane transport, oxidative stress, spore formation, and aflatoxin production. The MFS transporter genes AFLA_106900 (<i>mfs1</i>) and AFLA_106910 (<i>mfs2</i>) were crucial for an inhibitory effect of rPINB. The mutants exhibited reduced sensitivity to rPINB-mediated inhibition, indicating lower growth, sunken conidia, and shriveled hyphae, compared to the wild-type strain. The results also demonstrated decreased sensitivity to the stress agents affecting cell membranes, osmotic balance, and oxidation, alongside a significant reduction in AFB1 production in gene-deleted strains. These results suggested that <i>mfs1</i> and <i>mfs2</i> were essential for rPINB protein’s inhibition of <i>A. flavus</i> growth, laying the groundwork for the mold control strategies using plant proteins.
ISSN:2304-8158

Similar Items