Modulation of the gut-bone axis: Lacticaseibacillus paracasei LC86 improves bone health via anti-inflammatory metabolic pathways in zebrafish models of osteoporosis and cartilage damage
AimOsteoporosis and cartilage injury are major health concerns with limited treatment options. This study investigates the therapeutic effects of Lacticaseibacillus paracasei LC86 (LC86) on osteoporosis and cartilage damage in a zebrafish (Danio rerio) model, focusing on its modulation of the gut-bo...
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Frontiers Media S.A.
2025-04-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1493560/full |
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| author | Yao Dong Yao Dong Yukun Sun Zhipeng Zhou Zhonghui Gai Yihui Cai Mei Han Kang Zou Kang Zou |
| author_facet | Yao Dong Yao Dong Yukun Sun Zhipeng Zhou Zhonghui Gai Yihui Cai Mei Han Kang Zou Kang Zou |
| author_sort | Yao Dong |
| collection | DOAJ |
| description | AimOsteoporosis and cartilage injury are major health concerns with limited treatment options. This study investigates the therapeutic effects of Lacticaseibacillus paracasei LC86 (LC86) on osteoporosis and cartilage damage in a zebrafish (Danio rerio) model, focusing on its modulation of the gut-bone axis and its potential mechanisms for enhancing bone health.MethodsA Dexamethasone-induced zebrafish model was used to mimic osteoporosis and cartilage injury. Zebrafish were divided into control, model, and LC86 treatment groups (3×107 CFU/mL). Bone and cartilage health were assessed using Alizarin red staining and fluorescence microscopy. Bone marker expression (sp7, runx2a, bmp2a, bmp4, and col2a1a) was quantified via qPCR. Metabolic alterations were analyzed using untargeted metabolomics, and changes in gut microbiota were examined through 16S rRNA gene sequencing.ResultsLC86 treatment significantly improved bone and cartilage health, as evidenced by increased fluorescence intensity in the skull, hard bone, and cartilage (p < 0.01, p < 0.05). qPCR results showed upregulation of key bone-related genes (sp7, runx2a, bmp2a, bmp4, and col2a1a), indicating enhanced bone and cartilage structure. Metabolomics analysis revealed alterations in over 300 metabolites, with changes in anti-inflammatory and energy pathways. Gut microbiota analysis demonstrated an increase in beneficial bacteria and a decrease in pathogenic genera.ConclusionsLC86 significantly improved bone health, cartilage structure, and gut microbiota composition in a Dexamethasone-induced zebrafish model, supporting its potential as a therapeutic strategy for osteoporosis and cartilage injury via modulation of the gut-bone axis. |
| format | Article |
| id | doaj-art-abcfd629bcf74a35b71dad048b9d08d8 |
| institution | OA Journals |
| issn | 1664-3224 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj-art-abcfd629bcf74a35b71dad048b9d08d82025-08-20T02:26:23ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-04-011610.3389/fimmu.2025.14935601493560Modulation of the gut-bone axis: Lacticaseibacillus paracasei LC86 improves bone health via anti-inflammatory metabolic pathways in zebrafish models of osteoporosis and cartilage damageYao Dong0Yao Dong1Yukun Sun2Zhipeng Zhou3Zhonghui Gai4Yihui Cai5Mei Han6Kang Zou7Kang Zou8Germline Stem Cells and Microenvironment Lab, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, ChinaStem Cell Research and Translation Center, Nanjing Agricultural University, Nanjing, ChinaDepartment of Research and Development, Wecare Probiotics Co., Ltd., Suzhou, ChinaFood Science and Nutrition, University of Leeds, Leeds, United KingdomDepartment of Research and Development, Wecare Probiotics Co., Ltd., Suzhou, ChinaSchool of Biomedical Engineering, Hubei University of Medicine, Shiyan, ChinaDepartment of Food Quality and Safety, Shanghai Business School, Shanghai, ChinaGermline Stem Cells and Microenvironment Lab, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, ChinaStem Cell Research and Translation Center, Nanjing Agricultural University, Nanjing, ChinaAimOsteoporosis and cartilage injury are major health concerns with limited treatment options. This study investigates the therapeutic effects of Lacticaseibacillus paracasei LC86 (LC86) on osteoporosis and cartilage damage in a zebrafish (Danio rerio) model, focusing on its modulation of the gut-bone axis and its potential mechanisms for enhancing bone health.MethodsA Dexamethasone-induced zebrafish model was used to mimic osteoporosis and cartilage injury. Zebrafish were divided into control, model, and LC86 treatment groups (3×107 CFU/mL). Bone and cartilage health were assessed using Alizarin red staining and fluorescence microscopy. Bone marker expression (sp7, runx2a, bmp2a, bmp4, and col2a1a) was quantified via qPCR. Metabolic alterations were analyzed using untargeted metabolomics, and changes in gut microbiota were examined through 16S rRNA gene sequencing.ResultsLC86 treatment significantly improved bone and cartilage health, as evidenced by increased fluorescence intensity in the skull, hard bone, and cartilage (p < 0.01, p < 0.05). qPCR results showed upregulation of key bone-related genes (sp7, runx2a, bmp2a, bmp4, and col2a1a), indicating enhanced bone and cartilage structure. Metabolomics analysis revealed alterations in over 300 metabolites, with changes in anti-inflammatory and energy pathways. Gut microbiota analysis demonstrated an increase in beneficial bacteria and a decrease in pathogenic genera.ConclusionsLC86 significantly improved bone health, cartilage structure, and gut microbiota composition in a Dexamethasone-induced zebrafish model, supporting its potential as a therapeutic strategy for osteoporosis and cartilage injury via modulation of the gut-bone axis.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1493560/fullosteoporosiscartilage injuryLacticaseibacillus paracaseigut-bone axiszebrafish model |
| spellingShingle | Yao Dong Yao Dong Yukun Sun Zhipeng Zhou Zhonghui Gai Yihui Cai Mei Han Kang Zou Kang Zou Modulation of the gut-bone axis: Lacticaseibacillus paracasei LC86 improves bone health via anti-inflammatory metabolic pathways in zebrafish models of osteoporosis and cartilage damage Frontiers in Immunology osteoporosis cartilage injury Lacticaseibacillus paracasei gut-bone axis zebrafish model |
| title | Modulation of the gut-bone axis: Lacticaseibacillus paracasei LC86 improves bone health via anti-inflammatory metabolic pathways in zebrafish models of osteoporosis and cartilage damage |
| title_full | Modulation of the gut-bone axis: Lacticaseibacillus paracasei LC86 improves bone health via anti-inflammatory metabolic pathways in zebrafish models of osteoporosis and cartilage damage |
| title_fullStr | Modulation of the gut-bone axis: Lacticaseibacillus paracasei LC86 improves bone health via anti-inflammatory metabolic pathways in zebrafish models of osteoporosis and cartilage damage |
| title_full_unstemmed | Modulation of the gut-bone axis: Lacticaseibacillus paracasei LC86 improves bone health via anti-inflammatory metabolic pathways in zebrafish models of osteoporosis and cartilage damage |
| title_short | Modulation of the gut-bone axis: Lacticaseibacillus paracasei LC86 improves bone health via anti-inflammatory metabolic pathways in zebrafish models of osteoporosis and cartilage damage |
| title_sort | modulation of the gut bone axis lacticaseibacillus paracasei lc86 improves bone health via anti inflammatory metabolic pathways in zebrafish models of osteoporosis and cartilage damage |
| topic | osteoporosis cartilage injury Lacticaseibacillus paracasei gut-bone axis zebrafish model |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1493560/full |
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