Isolation of high molecular weight DNA suitable for BAC library construction from woody perennial soft-fruit species
We have developed a novel nuclei extraction method that allows for the extraction of high molecular weight DNA from leaves of woody perennial soft-fruit species that contain high levels of carbohydrates and polyphenolics. The method utilizes a modified buffer system including 4% (w/v) polyvinylpyrro...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2005-01-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/05381ST02 |
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