Sea cucumber gut tetrapeptide ameliorates alcoholic gastric damage via restoring mitochondrial dynamics
This paper aimed to explore the mechanism of tetrapeptide Val-Thr-Pro-Tyr (VTPY) in improving alcoholic gastric injury. VTPY has the potential to enhance the growth and movement of normal human gastric epithelial cells (GES-1). Following ethanol-induced impairment, VTPY effectively improved migratio...
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Tsinghua University Press
2025-05-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2024.9250183 |
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| author | Zhihong Zheng Na Sun Jingqi Yang Zhijie Bao Songyi Lin |
| author_facet | Zhihong Zheng Na Sun Jingqi Yang Zhijie Bao Songyi Lin |
| author_sort | Zhihong Zheng |
| collection | DOAJ |
| description | This paper aimed to explore the mechanism of tetrapeptide Val-Thr-Pro-Tyr (VTPY) in improving alcoholic gastric injury. VTPY has the potential to enhance the growth and movement of normal human gastric epithelial cells (GES-1). Following ethanol-induced impairment, VTPY effectively improved migration of GES-1 and human umbilical vein endothelial cells (HUVEC) cells, enhanced angiogenesis, eliminated cellular and mitochondrial reactive oxygen species (ROS), inhibited excessive mitochondrial division, enhanced F-actin polymerization and mitochondrial respiratory capacity. To counteract excessive mitochondrial fission, VTPY primarily restores the mitochondria dynamics by reducing the expression of Drp1 and Fis1, while increasing Mfn2. Further studies utilizing inhibitors clarifies that the inhibition of excessive mitochondrial fission can markedly reduce F-actin depolymerization, consequently enhancing cell migration. Additionally, VTPY can inhibit the apoptosis pathway by maintaining potential of mitochondrial membrane, preventing the release of mitochondrial cytochrome c, bolstering the levels of Bcl-XL, while reducing the levels of Bax and cleaved-caspase-3. Further investigations using inhibitors demonstrates that excessive mitochondrial fission could activate apoptotic pathway. However, VTPY counteracts this effect and enhance cells viability. Further evidence suggests that VTPY effectively improves ulcer index and pathologic changes, relieves inflammation, enhances the balance of oxidation and anti-oxidation, promotes angiogenesis, improves the expression of mitochondrial dynamics factors, blocks apoptotic pathway, and subsequently ameliorates gastric damage in mice through Fis-1/Bcl-2 pathway. |
| format | Article |
| id | doaj-art-a8f196b6f49e4ff0bbc5dc045adb2a6b |
| institution | DOAJ |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Tsinghua University Press |
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| series | Food Science and Human Wellness |
| spelling | doaj-art-a8f196b6f49e4ff0bbc5dc045adb2a6b2025-08-20T03:05:42ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302025-05-01145925018310.26599/FSHW.2024.9250183Sea cucumber gut tetrapeptide ameliorates alcoholic gastric damage via restoring mitochondrial dynamicsZhihong Zheng0Na Sun1Jingqi Yang2Zhijie Bao3Songyi Lin4State Key Laboratory of Marine Food Processing and Safety Control, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaState Key Laboratory of Marine Food Processing and Safety Control, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaState Key Laboratory of Marine Food Processing and Safety Control, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaState Key Laboratory of Marine Food Processing and Safety Control, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaState Key Laboratory of Marine Food Processing and Safety Control, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaThis paper aimed to explore the mechanism of tetrapeptide Val-Thr-Pro-Tyr (VTPY) in improving alcoholic gastric injury. VTPY has the potential to enhance the growth and movement of normal human gastric epithelial cells (GES-1). Following ethanol-induced impairment, VTPY effectively improved migration of GES-1 and human umbilical vein endothelial cells (HUVEC) cells, enhanced angiogenesis, eliminated cellular and mitochondrial reactive oxygen species (ROS), inhibited excessive mitochondrial division, enhanced F-actin polymerization and mitochondrial respiratory capacity. To counteract excessive mitochondrial fission, VTPY primarily restores the mitochondria dynamics by reducing the expression of Drp1 and Fis1, while increasing Mfn2. Further studies utilizing inhibitors clarifies that the inhibition of excessive mitochondrial fission can markedly reduce F-actin depolymerization, consequently enhancing cell migration. Additionally, VTPY can inhibit the apoptosis pathway by maintaining potential of mitochondrial membrane, preventing the release of mitochondrial cytochrome c, bolstering the levels of Bcl-XL, while reducing the levels of Bax and cleaved-caspase-3. Further investigations using inhibitors demonstrates that excessive mitochondrial fission could activate apoptotic pathway. However, VTPY counteracts this effect and enhance cells viability. Further evidence suggests that VTPY effectively improves ulcer index and pathologic changes, relieves inflammation, enhances the balance of oxidation and anti-oxidation, promotes angiogenesis, improves the expression of mitochondrial dynamics factors, blocks apoptotic pathway, and subsequently ameliorates gastric damage in mice through Fis-1/Bcl-2 pathway.https://www.sciopen.com/article/10.26599/FSHW.2024.9250183sea cucumber peptidemitochondrial fissioncell migrationethanolic gastric mucosa damageangiogenesis |
| spellingShingle | Zhihong Zheng Na Sun Jingqi Yang Zhijie Bao Songyi Lin Sea cucumber gut tetrapeptide ameliorates alcoholic gastric damage via restoring mitochondrial dynamics Food Science and Human Wellness sea cucumber peptide mitochondrial fission cell migration ethanolic gastric mucosa damage angiogenesis |
| title | Sea cucumber gut tetrapeptide ameliorates alcoholic gastric damage via restoring mitochondrial dynamics |
| title_full | Sea cucumber gut tetrapeptide ameliorates alcoholic gastric damage via restoring mitochondrial dynamics |
| title_fullStr | Sea cucumber gut tetrapeptide ameliorates alcoholic gastric damage via restoring mitochondrial dynamics |
| title_full_unstemmed | Sea cucumber gut tetrapeptide ameliorates alcoholic gastric damage via restoring mitochondrial dynamics |
| title_short | Sea cucumber gut tetrapeptide ameliorates alcoholic gastric damage via restoring mitochondrial dynamics |
| title_sort | sea cucumber gut tetrapeptide ameliorates alcoholic gastric damage via restoring mitochondrial dynamics |
| topic | sea cucumber peptide mitochondrial fission cell migration ethanolic gastric mucosa damage angiogenesis |
| url | https://www.sciopen.com/article/10.26599/FSHW.2024.9250183 |
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