A Citrus bergamia Extract Decreases Adipogenesis and Increases Lipolysis by Modulating PPAR Levels in Mesenchymal Stem Cells from Human Adipose Tissue
The aim of this research was to assess the impact of a well-characterized extract from Citrus bergamia juice on adipogenesis and/or lipolysis using mesenchymal stem cells from human adipose tissue as a cell model. To evaluate the effects on adipogenesis, some cell cultures were treated with adipogen...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | PPAR Research |
| Online Access: | http://dx.doi.org/10.1155/2016/4563815 |
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| author | Debora Lo Furno Adriana Carol Eleonora Graziano Rosanna Avola Rosario Giuffrida Vincenzo Perciavalle Francesco Bonina Giuliana Mannino Venera Cardile |
| author_facet | Debora Lo Furno Adriana Carol Eleonora Graziano Rosanna Avola Rosario Giuffrida Vincenzo Perciavalle Francesco Bonina Giuliana Mannino Venera Cardile |
| author_sort | Debora Lo Furno |
| collection | DOAJ |
| description | The aim of this research was to assess the impact of a well-characterized extract from Citrus bergamia juice on adipogenesis and/or lipolysis using mesenchymal stem cells from human adipose tissue as a cell model. To evaluate the effects on adipogenesis, some cell cultures were treated with adipogenic medium plus 10 or 100 μg/mL of extract. To determine the properties on lipolysis, additional mesenchymal stem cells were cultured with adipogenic medium for 14 days and after this time added with Citrus bergamia for further 14 days. To verify adipogenic differentiation, oil red O staining at 7, 14, 21, and 28 days was performed. Moreover, the expression of peroxisome proliferator-activated receptor gamma (PPAR-γ), adipocytes fatty acid-binding protein (A-FABP), adipose triglyceride lipase (ATGL), hormone-sensitive lipase (HSL), monoglyceride lipase (MGL), 5′-adenosine monophosphate-activated protein kinase (AMPK)α1/2, and pAMPKα1/2 was evaluated by Western blot analysis and the release of glycerol by colorimetric assay. Citrus bergamia extract suppressed the accumulation of intracellular lipids in mesenchymal stem cells during adipogenic differentiation and promoted lipolysis by repressing the expression of adipogenic genes and activating lipolytic genes. Citrus bergamia extract could be a useful natural product for improving adipose mobilization in obesity-related disorders. |
| format | Article |
| id | doaj-art-a854bb7a9b924c3ab05f6d8c4ad61fb5 |
| institution | Kabale University |
| issn | 1687-4757 1687-4765 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | PPAR Research |
| spelling | doaj-art-a854bb7a9b924c3ab05f6d8c4ad61fb52025-02-03T06:01:55ZengWileyPPAR Research1687-47571687-47652016-01-01201610.1155/2016/45638154563815A Citrus bergamia Extract Decreases Adipogenesis and Increases Lipolysis by Modulating PPAR Levels in Mesenchymal Stem Cells from Human Adipose TissueDebora Lo Furno0Adriana Carol Eleonora Graziano1Rosanna Avola2Rosario Giuffrida3Vincenzo Perciavalle4Francesco Bonina5Giuliana Mannino6Venera Cardile7Department of Biomedical and Biotechnological Sciences, Section of Physiology, University of Catania, Via S. Sofia 65, 95125 Catania, ItalyDepartment of Biomedical and Biotechnological Sciences, Section of Physiology, University of Catania, Via S. Sofia 65, 95125 Catania, ItalyDepartment of Biomedical and Biotechnological Sciences, Section of Physiology, University of Catania, Via S. Sofia 65, 95125 Catania, ItalyDepartment of Biomedical and Biotechnological Sciences, Section of Physiology, University of Catania, Via S. Sofia 65, 95125 Catania, ItalyDepartment of Biomedical and Biotechnological Sciences, Section of Physiology, University of Catania, Via S. Sofia 65, 95125 Catania, ItalyDepartment of Drug Sciences, University of Catania, Viale A. Doria 6, 95125 Catania, ItalyDepartment of Biomedical and Biotechnological Sciences, Section of Physiology, University of Catania, Via S. Sofia 65, 95125 Catania, ItalyDepartment of Biomedical and Biotechnological Sciences, Section of Physiology, University of Catania, Via S. Sofia 65, 95125 Catania, ItalyThe aim of this research was to assess the impact of a well-characterized extract from Citrus bergamia juice on adipogenesis and/or lipolysis using mesenchymal stem cells from human adipose tissue as a cell model. To evaluate the effects on adipogenesis, some cell cultures were treated with adipogenic medium plus 10 or 100 μg/mL of extract. To determine the properties on lipolysis, additional mesenchymal stem cells were cultured with adipogenic medium for 14 days and after this time added with Citrus bergamia for further 14 days. To verify adipogenic differentiation, oil red O staining at 7, 14, 21, and 28 days was performed. Moreover, the expression of peroxisome proliferator-activated receptor gamma (PPAR-γ), adipocytes fatty acid-binding protein (A-FABP), adipose triglyceride lipase (ATGL), hormone-sensitive lipase (HSL), monoglyceride lipase (MGL), 5′-adenosine monophosphate-activated protein kinase (AMPK)α1/2, and pAMPKα1/2 was evaluated by Western blot analysis and the release of glycerol by colorimetric assay. Citrus bergamia extract suppressed the accumulation of intracellular lipids in mesenchymal stem cells during adipogenic differentiation and promoted lipolysis by repressing the expression of adipogenic genes and activating lipolytic genes. Citrus bergamia extract could be a useful natural product for improving adipose mobilization in obesity-related disorders.http://dx.doi.org/10.1155/2016/4563815 |
| spellingShingle | Debora Lo Furno Adriana Carol Eleonora Graziano Rosanna Avola Rosario Giuffrida Vincenzo Perciavalle Francesco Bonina Giuliana Mannino Venera Cardile A Citrus bergamia Extract Decreases Adipogenesis and Increases Lipolysis by Modulating PPAR Levels in Mesenchymal Stem Cells from Human Adipose Tissue PPAR Research |
| title | A Citrus bergamia Extract Decreases Adipogenesis and Increases Lipolysis by Modulating PPAR Levels in Mesenchymal Stem Cells from Human Adipose Tissue |
| title_full | A Citrus bergamia Extract Decreases Adipogenesis and Increases Lipolysis by Modulating PPAR Levels in Mesenchymal Stem Cells from Human Adipose Tissue |
| title_fullStr | A Citrus bergamia Extract Decreases Adipogenesis and Increases Lipolysis by Modulating PPAR Levels in Mesenchymal Stem Cells from Human Adipose Tissue |
| title_full_unstemmed | A Citrus bergamia Extract Decreases Adipogenesis and Increases Lipolysis by Modulating PPAR Levels in Mesenchymal Stem Cells from Human Adipose Tissue |
| title_short | A Citrus bergamia Extract Decreases Adipogenesis and Increases Lipolysis by Modulating PPAR Levels in Mesenchymal Stem Cells from Human Adipose Tissue |
| title_sort | citrus bergamia extract decreases adipogenesis and increases lipolysis by modulating ppar levels in mesenchymal stem cells from human adipose tissue |
| url | http://dx.doi.org/10.1155/2016/4563815 |
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