Activation of cannabinoid type 2 (CB2) receptors promotes the maintenance of redox homeostasis and protects against oxidative distress in the Neotropical freshwater fish matrinxã Brycon amazonicus (Characiformes: Bryconidae)
Abstract Recent evidence indicates significant interaction of cannabinoid receptors (CB1 and CB2) with redox mechanisms. This study investigated the effects of the cannabinoid agonists WIN 55,212-2 (CB1+CB2) and HU-308 (CB2) on oxidative biomarkers in the liver and heart of the fish Brycon amazonicu...
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Sociedade Brasileira de Ictiologia
2024-11-01
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| Series: | Neotropical Ichthyology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252024000400202&lng=en&tlng=en |
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| author | Suzana Luisa Alves Fernandes Yan Costa Gonçalves Francisco Tadeu Rantin Ana Lúcia Kalinin Diana Amaral Monteiro |
| author_facet | Suzana Luisa Alves Fernandes Yan Costa Gonçalves Francisco Tadeu Rantin Ana Lúcia Kalinin Diana Amaral Monteiro |
| author_sort | Suzana Luisa Alves Fernandes |
| collection | DOAJ |
| description | Abstract Recent evidence indicates significant interaction of cannabinoid receptors (CB1 and CB2) with redox mechanisms. This study investigated the effects of the cannabinoid agonists WIN 55,212-2 (CB1+CB2) and HU-308 (CB2) on oxidative biomarkers in the liver and heart of the fish Brycon amazonicus. In both the liver and the heart, CB1+CB2 activation led to significant increases in catalase (CAT) and glutathione peroxidase (GPx) activities, accompanied by decreases in glutathione reductase (GR) activity. In contrast, glutathione S-transferase (GST) activity increased in the liver and decreased in the heart following CB1+CB2 activation. In addition, CB1+CB2 agonist had no effect on the GSH/GSSG ratio but increased heart levels of lipoperoxidation (LPO) and hepatic and cardiac protein carbonyl (PC) content. On the other hand, CB2 activation preserved antioxidant enzymatic activities and increased the GSH/GSSG ratio in both tissues. Moreover, the CB2 agonist showed no significant effect on PC levels in either tissue or cardiac LPO levels but decreased hepatic LPO content. In conclusion, activation of CB1+CB2 receptors disrupted the redox balance, leading to oxidative distress and damage, whereas activation of CB2 preserved oxidative eustress. These findings highlight the potential of CB2 receptors to modulate antioxidant defenses and maintain redox homeostasis, critical for improving fish health. |
| format | Article |
| id | doaj-art-a83ec1a95a4944aeb9855350687ca448 |
| institution | Kabale University |
| issn | 1982-0224 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Sociedade Brasileira de Ictiologia |
| record_format | Article |
| series | Neotropical Ichthyology |
| spelling | doaj-art-a83ec1a95a4944aeb9855350687ca4482025-01-21T07:40:01ZengSociedade Brasileira de IctiologiaNeotropical Ichthyology1982-02242024-11-0122410.1590/1982-0224-2024-0065Activation of cannabinoid type 2 (CB2) receptors promotes the maintenance of redox homeostasis and protects against oxidative distress in the Neotropical freshwater fish matrinxã Brycon amazonicus (Characiformes: Bryconidae)Suzana Luisa Alves Fernandeshttps://orcid.org/0000-0002-7458-2336Yan Costa Gonçalveshttps://orcid.org/0000-0002-2839-0421Francisco Tadeu Rantinhttps://orcid.org/0000-0002-3697-278XAna Lúcia Kalininhttps://orcid.org/0000-0002-5957-6472Diana Amaral Monteirohttps://orcid.org/0000-0002-1178-6673Abstract Recent evidence indicates significant interaction of cannabinoid receptors (CB1 and CB2) with redox mechanisms. This study investigated the effects of the cannabinoid agonists WIN 55,212-2 (CB1+CB2) and HU-308 (CB2) on oxidative biomarkers in the liver and heart of the fish Brycon amazonicus. In both the liver and the heart, CB1+CB2 activation led to significant increases in catalase (CAT) and glutathione peroxidase (GPx) activities, accompanied by decreases in glutathione reductase (GR) activity. In contrast, glutathione S-transferase (GST) activity increased in the liver and decreased in the heart following CB1+CB2 activation. In addition, CB1+CB2 agonist had no effect on the GSH/GSSG ratio but increased heart levels of lipoperoxidation (LPO) and hepatic and cardiac protein carbonyl (PC) content. On the other hand, CB2 activation preserved antioxidant enzymatic activities and increased the GSH/GSSG ratio in both tissues. Moreover, the CB2 agonist showed no significant effect on PC levels in either tissue or cardiac LPO levels but decreased hepatic LPO content. In conclusion, activation of CB1+CB2 receptors disrupted the redox balance, leading to oxidative distress and damage, whereas activation of CB2 preserved oxidative eustress. These findings highlight the potential of CB2 receptors to modulate antioxidant defenses and maintain redox homeostasis, critical for improving fish health.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252024000400202&lng=en&tlng=enAntioxidant defense systemCannabinoid agonistsHeartLiverOxidative damage |
| spellingShingle | Suzana Luisa Alves Fernandes Yan Costa Gonçalves Francisco Tadeu Rantin Ana Lúcia Kalinin Diana Amaral Monteiro Activation of cannabinoid type 2 (CB2) receptors promotes the maintenance of redox homeostasis and protects against oxidative distress in the Neotropical freshwater fish matrinxã Brycon amazonicus (Characiformes: Bryconidae) Neotropical Ichthyology Antioxidant defense system Cannabinoid agonists Heart Liver Oxidative damage |
| title | Activation of cannabinoid type 2 (CB2) receptors promotes the maintenance of redox homeostasis and protects against oxidative distress in the Neotropical freshwater fish matrinxã Brycon amazonicus (Characiformes: Bryconidae) |
| title_full | Activation of cannabinoid type 2 (CB2) receptors promotes the maintenance of redox homeostasis and protects against oxidative distress in the Neotropical freshwater fish matrinxã Brycon amazonicus (Characiformes: Bryconidae) |
| title_fullStr | Activation of cannabinoid type 2 (CB2) receptors promotes the maintenance of redox homeostasis and protects against oxidative distress in the Neotropical freshwater fish matrinxã Brycon amazonicus (Characiformes: Bryconidae) |
| title_full_unstemmed | Activation of cannabinoid type 2 (CB2) receptors promotes the maintenance of redox homeostasis and protects against oxidative distress in the Neotropical freshwater fish matrinxã Brycon amazonicus (Characiformes: Bryconidae) |
| title_short | Activation of cannabinoid type 2 (CB2) receptors promotes the maintenance of redox homeostasis and protects against oxidative distress in the Neotropical freshwater fish matrinxã Brycon amazonicus (Characiformes: Bryconidae) |
| title_sort | activation of cannabinoid type 2 cb2 receptors promotes the maintenance of redox homeostasis and protects against oxidative distress in the neotropical freshwater fish matrinxa brycon amazonicus characiformes bryconidae |
| topic | Antioxidant defense system Cannabinoid agonists Heart Liver Oxidative damage |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252024000400202&lng=en&tlng=en |
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