Characterization and validation of a middle-down hydrophobic interaction chromatography method to monitor methionine oxidation in IgG1

Post-translational modifications (PTMs) of therapeutic monoclonal antibodies (mAbs) can impact the efficacy of a drug. Methionine oxidation can alter the overall hydrophobicity of an antibody, thereby inducing conformational changes and affecting its biological activity. To ensure high quality, safe...

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Bibliographic Details
Main Authors: Somar Khalil, Nisha Patel, Francoise Bevillard-Kumar, Cyrille Chéry, William Burkitt, John O’Hara, Annick Gervais
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:Journal of Pharmaceutical and Biomedical Analysis Open
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Online Access:http://www.sciencedirect.com/science/article/pii/S2949771X23000087
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