Tracing the evolutionary trajectory of the IncP-2 plasmid co-harboring blaIMP-45 and blaVIM-1: an outbreak of Pseudomonas aeruginosa co-producing IMP-45 and VIM-1 carbapenemases in China
BackgroundCarbapenem-resistant Pseudomonas aeruginosa (CRPA) poses a significant global health risk, particularly for immunocompromised individuals. This study documents an outbreak of CRPA strains co-harboring blaVIM-1 and blaIMP-45 on IncP-2 plasmids in a Chinese tertiary hospital, resulting in po...
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Frontiers Media S.A.
2025-06-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| author | Yiqun Ma Yiqun Ma Yiqun Ma Zichen Lei Zichen Lei Zichen Lei Yulin Zhang Yulin Zhang Qi Liu Qi Liu Qi Liu Feilong Zhang Feilong Zhang Feilong Zhang Hao Zu Xinrui Yang Xinrui Yang Xinrui Yang Ziyao Li Ziyao Li Ziyao Li Binghuai Lu Binghuai Lu Binghuai Lu Binghuai Lu Binghuai Lu |
| author_facet | Yiqun Ma Yiqun Ma Yiqun Ma Zichen Lei Zichen Lei Zichen Lei Yulin Zhang Yulin Zhang Qi Liu Qi Liu Qi Liu Feilong Zhang Feilong Zhang Feilong Zhang Hao Zu Xinrui Yang Xinrui Yang Xinrui Yang Ziyao Li Ziyao Li Ziyao Li Binghuai Lu Binghuai Lu Binghuai Lu Binghuai Lu Binghuai Lu |
| author_sort | Yiqun Ma |
| collection | DOAJ |
| description | BackgroundCarbapenem-resistant Pseudomonas aeruginosa (CRPA) poses a significant global health risk, particularly for immunocompromised individuals. This study documents an outbreak of CRPA strains co-harboring blaVIM-1 and blaIMP-45 on IncP-2 plasmids in a Chinese tertiary hospital, resulting in poor outcomes for transplant patients.Methods17 ST313 VIM-1-IMP-45 CRPA strains were collected from transplant patients, and antibiotic susceptibility was tested via microbroth dilution. Whole genome sequencing (WGS) identified drug resistance and virulence mechanisms, analyzed ST313 P. aeruginosa phylogeny, and traced blaVIM-1 and blaIMP-45 origins. Conjugation experiments were conducted to assess the conjugative potential of the IncP-2 plasmid co-harboring blaVIM-1 and blaIMP-45. Structural and molecular docking studies explored the PBP3 (P527S) mutation’s role in aztreonam resistance.ResultsFrom February 2022 to July 2024, 17 ST313 VIM-1-IMP-45 CRPA strains from 10 transplant patients were identified. All strains were extensively drug-resistant but sensitive to colistin and cefiderocol. WGS showed blaIMP-45 and blaVIM-1 on an IncP-2 megaplasmid. Phylogenetic analysis indicated high homology with plasmids carrying blaIMP-45. Further analysis of the genetic environment showed that the IncP-2 plasmid co-harboring blaVIM-1 and blaIMP-45 was formed by the insertion of a Tn3-family transposon carrying blaVIM-1 into the IncP-2 plasmid carrying blaIMP-45. In addition aztreonam-resistant strains (14/15) had a PBP3 (P527S) mutation, with molecular docking studies suggesting reduced aztreonam binding.ConclusionsThis study reports a clonal outbreak of ST313 P. aeruginosa strains co-producing IMP-45 and VIM-1 carbapenemases in a tertiary hospital. The evolutionary path of the IncP-2 plasmid co-harboring blaIMP-45 and blaVIM-1 was elucidated. |
| format | Article |
| id | doaj-art-a6bc1d86e381476b93ce2e48a5f57073 |
| institution | DOAJ |
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| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-a6bc1d86e381476b93ce2e48a5f570732025-08-20T03:22:04ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-06-011510.3389/fcimb.2025.16232411623241Tracing the evolutionary trajectory of the IncP-2 plasmid co-harboring blaIMP-45 and blaVIM-1: an outbreak of Pseudomonas aeruginosa co-producing IMP-45 and VIM-1 carbapenemases in ChinaYiqun Ma0Yiqun Ma1Yiqun Ma2Zichen Lei3Zichen Lei4Zichen Lei5Yulin Zhang6Yulin Zhang7Qi Liu8Qi Liu9Qi Liu10Feilong Zhang11Feilong Zhang12Feilong Zhang13Hao Zu14Xinrui Yang15Xinrui Yang16Xinrui Yang17Ziyao Li18Ziyao Li19Ziyao Li20Binghuai Lu21Binghuai Lu22Binghuai Lu23Binghuai Lu24Binghuai Lu25Peking University China-Japan Friendship School of Clinical Medicine, China-Japan Friendship Hospital, Beijing, ChinaLaboratory of Clinical Microbiology and Infectious Diseases, Department of Pulmonary and Critical Care Medicine, Beijing Key Laboratory of Surveillance, Early Warning and Pathogen Research on Emerging Infectious Diseases, National Center for Respiratory Medicine, China-Japan Friendship Hospital, Beijing, ChinaInstitute of Respiratory Medicine, Chinese Academy of Medical Sciences, Beijing, ChinaLaboratory of Clinical Microbiology and Infectious Diseases, Department of Pulmonary and Critical Care Medicine, Beijing Key Laboratory of Surveillance, Early Warning and Pathogen Research on Emerging Infectious Diseases, National Center for Respiratory Medicine, China-Japan Friendship Hospital, Beijing, ChinaInstitute of Respiratory Medicine, Chinese Academy of Medical Sciences, Beijing, ChinaChina-Japan Friendship Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing, ChinaLaboratory of Clinical Microbiology and Infectious Diseases, Department of Pulmonary and Critical Care Medicine, Beijing Key Laboratory of Surveillance, Early Warning and Pathogen Research on Emerging Infectious Diseases, National Center for Respiratory Medicine, China-Japan Friendship Hospital, Beijing, ChinaInstitute of Respiratory Medicine, Chinese Academy of Medical Sciences, Beijing, ChinaLaboratory of Clinical Microbiology and Infectious Diseases, Department of Pulmonary and Critical Care Medicine, Beijing Key Laboratory of Surveillance, Early Warning and Pathogen Research on Emerging Infectious Diseases, National Center for Respiratory Medicine, China-Japan Friendship Hospital, Beijing, ChinaInstitute of Respiratory Medicine, Chinese Academy of Medical Sciences, Beijing, ChinaChina-Japan Friendship Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing, ChinaLaboratory of Clinical Microbiology and Infectious Diseases, Department of Pulmonary and Critical Care Medicine, Beijing Key Laboratory of Surveillance, Early Warning and Pathogen Research on Emerging Infectious Diseases, National Center for Respiratory Medicine, China-Japan Friendship Hospital, Beijing, ChinaInstitute of Respiratory Medicine, Chinese Academy of Medical Sciences, Beijing, ChinaPeking Union Medical College, Chinese Academy of Medical Sciences, Beijing, ChinaYanjing Medical College, Capital Medical University, Beijing, ChinaLaboratory of Clinical Microbiology and Infectious Diseases, Department of Pulmonary and Critical Care Medicine, Beijing Key Laboratory of Surveillance, Early Warning and Pathogen Research on Emerging Infectious Diseases, National Center for Respiratory Medicine, China-Japan Friendship Hospital, Beijing, ChinaInstitute of Respiratory Medicine, Chinese Academy of Medical Sciences, Beijing, ChinaPeking Union Medical College, Chinese Academy of Medical Sciences, Beijing, ChinaLaboratory of Clinical Microbiology and Infectious Diseases, Department of Pulmonary and Critical Care Medicine, Beijing Key Laboratory of Surveillance, Early Warning and Pathogen Research on Emerging Infectious Diseases, National Center for Respiratory Medicine, China-Japan Friendship Hospital, Beijing, ChinaInstitute of Respiratory Medicine, Chinese Academy of Medical Sciences, Beijing, ChinaPeking Union Medical College, Chinese Academy of Medical Sciences, Beijing, ChinaPeking University China-Japan Friendship School of Clinical Medicine, China-Japan Friendship Hospital, Beijing, ChinaLaboratory of Clinical Microbiology and Infectious Diseases, Department of Pulmonary and Critical Care Medicine, Beijing Key Laboratory of Surveillance, Early Warning and Pathogen Research on Emerging Infectious Diseases, National Center for Respiratory Medicine, China-Japan Friendship Hospital, Beijing, ChinaInstitute of Respiratory Medicine, Chinese Academy of Medical Sciences, Beijing, ChinaChina-Japan Friendship Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing, ChinaPeking Union Medical College, Chinese Academy of Medical Sciences, Beijing, ChinaBackgroundCarbapenem-resistant Pseudomonas aeruginosa (CRPA) poses a significant global health risk, particularly for immunocompromised individuals. This study documents an outbreak of CRPA strains co-harboring blaVIM-1 and blaIMP-45 on IncP-2 plasmids in a Chinese tertiary hospital, resulting in poor outcomes for transplant patients.Methods17 ST313 VIM-1-IMP-45 CRPA strains were collected from transplant patients, and antibiotic susceptibility was tested via microbroth dilution. Whole genome sequencing (WGS) identified drug resistance and virulence mechanisms, analyzed ST313 P. aeruginosa phylogeny, and traced blaVIM-1 and blaIMP-45 origins. Conjugation experiments were conducted to assess the conjugative potential of the IncP-2 plasmid co-harboring blaVIM-1 and blaIMP-45. Structural and molecular docking studies explored the PBP3 (P527S) mutation’s role in aztreonam resistance.ResultsFrom February 2022 to July 2024, 17 ST313 VIM-1-IMP-45 CRPA strains from 10 transplant patients were identified. All strains were extensively drug-resistant but sensitive to colistin and cefiderocol. WGS showed blaIMP-45 and blaVIM-1 on an IncP-2 megaplasmid. Phylogenetic analysis indicated high homology with plasmids carrying blaIMP-45. Further analysis of the genetic environment showed that the IncP-2 plasmid co-harboring blaVIM-1 and blaIMP-45 was formed by the insertion of a Tn3-family transposon carrying blaVIM-1 into the IncP-2 plasmid carrying blaIMP-45. In addition aztreonam-resistant strains (14/15) had a PBP3 (P527S) mutation, with molecular docking studies suggesting reduced aztreonam binding.ConclusionsThis study reports a clonal outbreak of ST313 P. aeruginosa strains co-producing IMP-45 and VIM-1 carbapenemases in a tertiary hospital. The evolutionary path of the IncP-2 plasmid co-harboring blaIMP-45 and blaVIM-1 was elucidated.https://www.frontiersin.org/articles/10.3389/fcimb.2025.1623241/fullcarbapenem-resistant Pseudomonas aeruginosaCRPAhorizontal gene transfer (HGT)nosocomial infectionmetallo-beta-lactamases (MBL)resistance dissemination |
| spellingShingle | Yiqun Ma Yiqun Ma Yiqun Ma Zichen Lei Zichen Lei Zichen Lei Yulin Zhang Yulin Zhang Qi Liu Qi Liu Qi Liu Feilong Zhang Feilong Zhang Feilong Zhang Hao Zu Xinrui Yang Xinrui Yang Xinrui Yang Ziyao Li Ziyao Li Ziyao Li Binghuai Lu Binghuai Lu Binghuai Lu Binghuai Lu Binghuai Lu Tracing the evolutionary trajectory of the IncP-2 plasmid co-harboring blaIMP-45 and blaVIM-1: an outbreak of Pseudomonas aeruginosa co-producing IMP-45 and VIM-1 carbapenemases in China Frontiers in Cellular and Infection Microbiology carbapenem-resistant Pseudomonas aeruginosa CRPA horizontal gene transfer (HGT) nosocomial infection metallo-beta-lactamases (MBL) resistance dissemination |
| title | Tracing the evolutionary trajectory of the IncP-2 plasmid co-harboring blaIMP-45 and blaVIM-1: an outbreak of Pseudomonas aeruginosa co-producing IMP-45 and VIM-1 carbapenemases in China |
| title_full | Tracing the evolutionary trajectory of the IncP-2 plasmid co-harboring blaIMP-45 and blaVIM-1: an outbreak of Pseudomonas aeruginosa co-producing IMP-45 and VIM-1 carbapenemases in China |
| title_fullStr | Tracing the evolutionary trajectory of the IncP-2 plasmid co-harboring blaIMP-45 and blaVIM-1: an outbreak of Pseudomonas aeruginosa co-producing IMP-45 and VIM-1 carbapenemases in China |
| title_full_unstemmed | Tracing the evolutionary trajectory of the IncP-2 plasmid co-harboring blaIMP-45 and blaVIM-1: an outbreak of Pseudomonas aeruginosa co-producing IMP-45 and VIM-1 carbapenemases in China |
| title_short | Tracing the evolutionary trajectory of the IncP-2 plasmid co-harboring blaIMP-45 and blaVIM-1: an outbreak of Pseudomonas aeruginosa co-producing IMP-45 and VIM-1 carbapenemases in China |
| title_sort | tracing the evolutionary trajectory of the incp 2 plasmid co harboring blaimp 45 and blavim 1 an outbreak of pseudomonas aeruginosa co producing imp 45 and vim 1 carbapenemases in china |
| topic | carbapenem-resistant Pseudomonas aeruginosa CRPA horizontal gene transfer (HGT) nosocomial infection metallo-beta-lactamases (MBL) resistance dissemination |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1623241/full |
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