In vitro cytotoxicity assessment of the 3D printed polymer based epoxy resin intended for use in dentistry
Background/Aim. There is limited published evidence on the cytotoxicity of 3D printed polymer materials for dentistry applications, despite that they are now being widely used in medicine. Stereolithography (SLA) is one of the foremost 3D processes used in 3D printing, yet there are only a...
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Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade
2019-01-01
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| Series: | Vojnosanitetski Pregled |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/0042-8450/2019/0042-84501700127P.pdf |
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| author | Puškar Tatjana Trifković Branka Đurović-Koprivica Daniela Kojić Vesna Jevremović Ana Mirković Siniša Eggbeer Dominic |
| author_facet | Puškar Tatjana Trifković Branka Đurović-Koprivica Daniela Kojić Vesna Jevremović Ana Mirković Siniša Eggbeer Dominic |
| author_sort | Puškar Tatjana |
| collection | DOAJ |
| description | Background/Aim. There is limited published evidence on the cytotoxicity of 3D
printed polymer materials for dentistry applications, despite that they are
now being widely used in medicine. Stereolithography (SLA) is one of the
foremost 3D processes used in 3D printing, yet there are only a small number
of resin materials reported to be suitable for medical applications. The aim
of this study was to investigate, in vitro, the cytotoxic effect of the 3D
printed resin in order to establish the suitability for its usage in
dentistry and related medical applications such as surgical dental guides,
occlusal splits and orthodontic devices. Methods. To examine the cytotoxicity
of the 3D printed polymer-based epoxy resin, Accura® ClearVue™ (3D-Systems,
USA), two cell cultures were used: mouse fibroblasts L929 and human lung
fibroblasts MRC-5. Cell viability was determined by Mosmann's colorimetric
(MTT) test and the agar diffusion test (ADT). Results. Direct contact of the
tested material with ADT test showed nontoxic effects of tested material in
any cell culture. The tested material showed no cytotoxic effect after 3 days
of extraction of the eluate by MTT test, but mild cytotoxic effect after 5, 7
and 21 days on both cell lines. The cytotoxicity increased with increasing
the time of the eluate extraction. Conclusion. 3D printed polymer-based epoxy
resin, Accura® ClearVue™ (3D-Systems, USA) is considered appropriate for
making surgical dental implant guides according to the cytotoxic behavior.
According to the mild level of cytotoxicity after the longer extraction
periods, there is a need for further evaluation of biocompatibility for its
application for occlusal splints and orthodontic devices. [Project of the
Serbian Ministry of Education, Science and Technological Development, Grant
no. TR 35020: Research and development of modeling methods and approaches in
manufacturing of dental recoveries with the application of modern
technologies and computer aided systems] |
| format | Article |
| id | doaj-art-a62b5a539fd845928b5d9c84f12c28f2 |
| institution | DOAJ |
| issn | 0042-8450 2406-0720 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade |
| record_format | Article |
| series | Vojnosanitetski Pregled |
| spelling | doaj-art-a62b5a539fd845928b5d9c84f12c28f22025-08-20T03:20:00ZengMinistry of Defence of the Republic of Serbia, University of Defence, BelgradeVojnosanitetski Pregled0042-84502406-07202019-01-0176550250910.2298/VSP170721127P0042-84501700127PIn vitro cytotoxicity assessment of the 3D printed polymer based epoxy resin intended for use in dentistryPuškar Tatjana0Trifković Branka1Đurović-Koprivica Daniela2Kojić Vesna3Jevremović Ana4Mirković Siniša5Eggbeer Dominic6University of Novi Sad, Faculty of Medicine, Department of Dentistry, Novi Sad, Serbia University of Belgrade, Faculty of Dentistry, Clinic for Prosthodontics, Belgrade, SerbiaUniversity of Novi Sad, Faculty of Medicine, Department of Dentistry, Novi Sad, Serbia University of Novi Sad, Faculty of Medicine, Oncology Institute of Vojvodina, Sremska Kamenica, Serbia University Business Academy in Novi Sad, Faculty of Dentistry, Pančevo, SerbiaUniversity of Novi Sad, Faculty of Medicine, Department of Dentistry, Novi Sad, SerbiaCardiff Metropolitan University, National Centre for Product Design and Development Research, Cardiff, United KingdomBackground/Aim. There is limited published evidence on the cytotoxicity of 3D printed polymer materials for dentistry applications, despite that they are now being widely used in medicine. Stereolithography (SLA) is one of the foremost 3D processes used in 3D printing, yet there are only a small number of resin materials reported to be suitable for medical applications. The aim of this study was to investigate, in vitro, the cytotoxic effect of the 3D printed resin in order to establish the suitability for its usage in dentistry and related medical applications such as surgical dental guides, occlusal splits and orthodontic devices. Methods. To examine the cytotoxicity of the 3D printed polymer-based epoxy resin, Accura® ClearVue™ (3D-Systems, USA), two cell cultures were used: mouse fibroblasts L929 and human lung fibroblasts MRC-5. Cell viability was determined by Mosmann's colorimetric (MTT) test and the agar diffusion test (ADT). Results. Direct contact of the tested material with ADT test showed nontoxic effects of tested material in any cell culture. The tested material showed no cytotoxic effect after 3 days of extraction of the eluate by MTT test, but mild cytotoxic effect after 5, 7 and 21 days on both cell lines. The cytotoxicity increased with increasing the time of the eluate extraction. Conclusion. 3D printed polymer-based epoxy resin, Accura® ClearVue™ (3D-Systems, USA) is considered appropriate for making surgical dental implant guides according to the cytotoxic behavior. According to the mild level of cytotoxicity after the longer extraction periods, there is a need for further evaluation of biocompatibility for its application for occlusal splints and orthodontic devices. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. TR 35020: Research and development of modeling methods and approaches in manufacturing of dental recoveries with the application of modern technologies and computer aided systems]http://www.doiserbia.nb.rs/img/doi/0042-8450/2019/0042-84501700127P.pdfcell culture techniquesdental materialsepoxy resinmaterial testingprinting, three-dimensional |
| spellingShingle | Puškar Tatjana Trifković Branka Đurović-Koprivica Daniela Kojić Vesna Jevremović Ana Mirković Siniša Eggbeer Dominic In vitro cytotoxicity assessment of the 3D printed polymer based epoxy resin intended for use in dentistry Vojnosanitetski Pregled cell culture techniques dental materials epoxy resin material testing printing, three-dimensional |
| title | In vitro cytotoxicity assessment of the 3D printed polymer based epoxy resin intended for use in dentistry |
| title_full | In vitro cytotoxicity assessment of the 3D printed polymer based epoxy resin intended for use in dentistry |
| title_fullStr | In vitro cytotoxicity assessment of the 3D printed polymer based epoxy resin intended for use in dentistry |
| title_full_unstemmed | In vitro cytotoxicity assessment of the 3D printed polymer based epoxy resin intended for use in dentistry |
| title_short | In vitro cytotoxicity assessment of the 3D printed polymer based epoxy resin intended for use in dentistry |
| title_sort | in vitro cytotoxicity assessment of the 3d printed polymer based epoxy resin intended for use in dentistry |
| topic | cell culture techniques dental materials epoxy resin material testing printing, three-dimensional |
| url | http://www.doiserbia.nb.rs/img/doi/0042-8450/2019/0042-84501700127P.pdf |
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