Mini-G proteins: Novel tools for studying GPCRs in their active conformation.

Mini-G proteins are the engineered GTPase domains of Gα subunits. They couple to GPCRs and recapitulate the increase in agonist affinity observed upon coupling of a native heterotrimeric G protein. Given the small size and stability of mini-G proteins, and their ease of expression and purification,...

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Bibliographic Details
Main Authors: Rony Nehmé, Byron Carpenter, Ankita Singhal, Annette Strege, Patricia C Edwards, Courtney F White, Haijuan Du, Reinhard Grisshammer, Christopher G Tate
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2017-01-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0175642&type=printable
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