Targeted sequencing of Enterobacterales bacteria using CRISPR-Cas9 enrichment and Oxford Nanopore Technologies

Targeted sequencing of Enterobacterales bacteria using CRISPR-Cas9 enrichment and Oxford Nanopore Technologies

ABSTRACT Sequencing DNA directly from patient samples enables faster pathogen characterization compared to traditional culture-based approaches, but often yields insufficient sequence data for effective downstream analysis. CRISPR-Cas9 enrichment is designed to improve the yield of low abundance seq...

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Main Authors: Hugh Cottingham, Louise M. Judd, Jessica A. Wisniewski, Ryan R. Wick, Thomas D. Stanton, Ben Vezina, Nenad Macesic, Anton Y. Peleg, Iruka N. Okeke, Kathryn E. Holt, Jane Hawkey
Format: Article
Language:English
Published: American Society for Microbiology 2025-02-01
Series:mSystems
Subjects:
Oxford Nanopore
CRISPR-Cas9 enrichment
Klebsiella
Enterobacterales
metagenomics
Online Access:https://journals.asm.org/doi/10.1128/msystems.01413-24
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https://journals.asm.org/doi/10.1128/msystems.01413-24

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