Molecular Identification and Antimicrobial Potential of Streptomyces Species from Nepalese Soil
Streptomyces are widely used for the production of secondary metabolites with diverse biological activities, including antibiotics. The necessity of alternative antimicrobial agents against multidrug-resistant pathogens is indispensable. However, the production of new therapeutics is delayed in rece...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2020/8817467 |
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| author | Karan Khadayat Dawa Dindu Sherpa Krishna Prakash Malla Sunil Shrestha Nabin Rana Bishnu P. Marasini Santosh Khanal Binod Rayamajhee Bibek Raj Bhattarai Niranjan Parajuli |
| author_facet | Karan Khadayat Dawa Dindu Sherpa Krishna Prakash Malla Sunil Shrestha Nabin Rana Bishnu P. Marasini Santosh Khanal Binod Rayamajhee Bibek Raj Bhattarai Niranjan Parajuli |
| author_sort | Karan Khadayat |
| collection | DOAJ |
| description | Streptomyces are widely used for the production of secondary metabolites with diverse biological activities, including antibiotics. The necessity of alternative antimicrobial agents against multidrug-resistant pathogens is indispensable. However, the production of new therapeutics is delayed in recent days. Thus, the isolation of new Streptomyces species has drawn attention. Nepal—a country rich in biodiversity—has got high possibilities for the discovery of members of actinomycetes, especially in the higher altitudes. However, in vain, only a few screening research works have been reported from Nepal to date. Streptomyces species were isolated on ISP4 media, and characterization was performed according to morphological similarity and 16S rRNA sequence similarity using bioinformatic tools. Ethyl acetate extracts of Streptomyces species were prepared, and the antimicrobial activity was carried out using agar well diffusion technique. In this report, 18 Streptomyces species isolated from the soil were reported based on sequence analysis of 16S rRNA. Among them, 12 isolates have shown antibacterial activity against extended-spectrum beta-lactamase- (ESBL-) producing Escherichia coli. Here, we have also analyzed 16S rRNA in 27 Streptomyces species whose whole-genome sequence is available, which has revealed that some species have multiple copies of the 16S gene (∼1.5 kb) with significant variation in nucleotides. In contrast, some Streptomyces species shared identical DNA sequences in multiple copies of 16S rRNA. The sequencing of numerous copies of 16S rRNA is not necessary, and the molecular sequencing of this region is not sufficient for the identification of bacterial species. The Streptomyces species-derived ethyl acetate extracts from Nepalese soil demonstrate potential activity against ESBL-producing E. coli. Thus, they are potential candidates for antibiotics manufacturing in the future. |
| format | Article |
| id | doaj-art-a5b14ff3f98749e7aaf5f8601755aaba |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-a5b14ff3f98749e7aaf5f8601755aaba2025-02-03T01:06:17ZengWileyInternational Journal of Microbiology1687-918X1687-91982020-01-01202010.1155/2020/88174678817467Molecular Identification and Antimicrobial Potential of Streptomyces Species from Nepalese SoilKaran Khadayat0Dawa Dindu Sherpa1Krishna Prakash Malla2Sunil Shrestha3Nabin Rana4Bishnu P. Marasini5Santosh Khanal6Binod Rayamajhee7Bibek Raj Bhattarai8Niranjan Parajuli9Central Department of Chemistry, Tribhuvan University, Kirtipur, Kathmandu, NepalDepartment of Biotechnology, National College, Tribhuvan University, Naya Bazar, Kathmandu, NepalDepartment of Biotechnology, National College, Tribhuvan University, Naya Bazar, Kathmandu, NepalDepartment of Biotechnology, National College, Tribhuvan University, Naya Bazar, Kathmandu, NepalDepartment of Biotechnology, National College, Tribhuvan University, Naya Bazar, Kathmandu, NepalDepartment of Biotechnology, National College, Tribhuvan University, Naya Bazar, Kathmandu, NepalDepartment of Microbiology, National College, Tribhuvan University, Naya Bazar, Kathmandu, NepalDepartment of Microbiology, National College, Tribhuvan University, Naya Bazar, Kathmandu, NepalCentral Department of Chemistry, Tribhuvan University, Kirtipur, Kathmandu, NepalCentral Department of Chemistry, Tribhuvan University, Kirtipur, Kathmandu, NepalStreptomyces are widely used for the production of secondary metabolites with diverse biological activities, including antibiotics. The necessity of alternative antimicrobial agents against multidrug-resistant pathogens is indispensable. However, the production of new therapeutics is delayed in recent days. Thus, the isolation of new Streptomyces species has drawn attention. Nepal—a country rich in biodiversity—has got high possibilities for the discovery of members of actinomycetes, especially in the higher altitudes. However, in vain, only a few screening research works have been reported from Nepal to date. Streptomyces species were isolated on ISP4 media, and characterization was performed according to morphological similarity and 16S rRNA sequence similarity using bioinformatic tools. Ethyl acetate extracts of Streptomyces species were prepared, and the antimicrobial activity was carried out using agar well diffusion technique. In this report, 18 Streptomyces species isolated from the soil were reported based on sequence analysis of 16S rRNA. Among them, 12 isolates have shown antibacterial activity against extended-spectrum beta-lactamase- (ESBL-) producing Escherichia coli. Here, we have also analyzed 16S rRNA in 27 Streptomyces species whose whole-genome sequence is available, which has revealed that some species have multiple copies of the 16S gene (∼1.5 kb) with significant variation in nucleotides. In contrast, some Streptomyces species shared identical DNA sequences in multiple copies of 16S rRNA. The sequencing of numerous copies of 16S rRNA is not necessary, and the molecular sequencing of this region is not sufficient for the identification of bacterial species. The Streptomyces species-derived ethyl acetate extracts from Nepalese soil demonstrate potential activity against ESBL-producing E. coli. Thus, they are potential candidates for antibiotics manufacturing in the future.http://dx.doi.org/10.1155/2020/8817467 |
| spellingShingle | Karan Khadayat Dawa Dindu Sherpa Krishna Prakash Malla Sunil Shrestha Nabin Rana Bishnu P. Marasini Santosh Khanal Binod Rayamajhee Bibek Raj Bhattarai Niranjan Parajuli Molecular Identification and Antimicrobial Potential of Streptomyces Species from Nepalese Soil International Journal of Microbiology |
| title | Molecular Identification and Antimicrobial Potential of Streptomyces Species from Nepalese Soil |
| title_full | Molecular Identification and Antimicrobial Potential of Streptomyces Species from Nepalese Soil |
| title_fullStr | Molecular Identification and Antimicrobial Potential of Streptomyces Species from Nepalese Soil |
| title_full_unstemmed | Molecular Identification and Antimicrobial Potential of Streptomyces Species from Nepalese Soil |
| title_short | Molecular Identification and Antimicrobial Potential of Streptomyces Species from Nepalese Soil |
| title_sort | molecular identification and antimicrobial potential of streptomyces species from nepalese soil |
| url | http://dx.doi.org/10.1155/2020/8817467 |
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