Protocol for adeno-associated virus-mediated gene delivery to accelerate pancreatic carcinogenesis in mice
Summary: Introducing additional genetic modifications or environmental stimuli accelerates early pancreatic carcinogenesis in the p48Cre/+; LSL-KrasG12D/+ (KC) mouse model. Here, we present a protocol employing adeno-associated virus (AAV)-mediated gene delivery specifically in the pancreas to facil...
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Elsevier
2025-09-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725003132 |
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| author | Hongzhen Li Zhao Shi Shanshan Shen Bo Kong |
| author_facet | Hongzhen Li Zhao Shi Shanshan Shen Bo Kong |
| author_sort | Hongzhen Li |
| collection | DOAJ |
| description | Summary: Introducing additional genetic modifications or environmental stimuli accelerates early pancreatic carcinogenesis in the p48Cre/+; LSL-KrasG12D/+ (KC) mouse model. Here, we present a protocol employing adeno-associated virus (AAV)-mediated gene delivery specifically in the pancreas to facilitate the progression from acinar cells through acinar-to-ductal metaplasia (ADM) to pancreatic intraepithelial neoplasia (PanIN). We describe steps for AAV production, preparing KC mice, intra-pancreatic AAV injection, and analysis. This protocol allows the investigation of early events of pancreatic carcinogenesis in a spatially and temporally controlled manner.For complete details on the use and execution of this protocol, please refer to Li et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-a5664a23c5d8482d8ea6ca2a0072692b |
| institution | DOAJ |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-a5664a23c5d8482d8ea6ca2a0072692b2025-08-20T03:21:46ZengElsevierSTAR Protocols2666-16672025-09-016310390710.1016/j.xpro.2025.103907Protocol for adeno-associated virus-mediated gene delivery to accelerate pancreatic carcinogenesis in miceHongzhen Li0Zhao Shi1Shanshan Shen2Bo Kong3Department of Gastroenterology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, Jiangsu Province, China; Department of General, Visceral and Transplantation Surgery, University of Heidelberg, Heidelberg, Germany; Corresponding authorDepartment of General, Visceral and Transplantation Surgery, University of Heidelberg, Heidelberg, Germany; Department of Gastroenterology, Nanjing Drum Tower Hospital, Clinical College of Nanjing Medical University, Nanjing, Jiangsu Province, ChinaDepartment of Gastroenterology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, Jiangsu Province, China; Department of Gastroenterology, Nanjing Drum Tower Hospital, Clinical College of Nanjing Medical University, Nanjing, Jiangsu Province, ChinaDepartment of General, Visceral and Transplantation Surgery, University of Heidelberg, Heidelberg, Germany; Corresponding authorSummary: Introducing additional genetic modifications or environmental stimuli accelerates early pancreatic carcinogenesis in the p48Cre/+; LSL-KrasG12D/+ (KC) mouse model. Here, we present a protocol employing adeno-associated virus (AAV)-mediated gene delivery specifically in the pancreas to facilitate the progression from acinar cells through acinar-to-ductal metaplasia (ADM) to pancreatic intraepithelial neoplasia (PanIN). We describe steps for AAV production, preparing KC mice, intra-pancreatic AAV injection, and analysis. This protocol allows the investigation of early events of pancreatic carcinogenesis in a spatially and temporally controlled manner.For complete details on the use and execution of this protocol, please refer to Li et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725003132cell culturecancerhealth sciences |
| spellingShingle | Hongzhen Li Zhao Shi Shanshan Shen Bo Kong Protocol for adeno-associated virus-mediated gene delivery to accelerate pancreatic carcinogenesis in mice STAR Protocols cell culture cancer health sciences |
| title | Protocol for adeno-associated virus-mediated gene delivery to accelerate pancreatic carcinogenesis in mice |
| title_full | Protocol for adeno-associated virus-mediated gene delivery to accelerate pancreatic carcinogenesis in mice |
| title_fullStr | Protocol for adeno-associated virus-mediated gene delivery to accelerate pancreatic carcinogenesis in mice |
| title_full_unstemmed | Protocol for adeno-associated virus-mediated gene delivery to accelerate pancreatic carcinogenesis in mice |
| title_short | Protocol for adeno-associated virus-mediated gene delivery to accelerate pancreatic carcinogenesis in mice |
| title_sort | protocol for adeno associated virus mediated gene delivery to accelerate pancreatic carcinogenesis in mice |
| topic | cell culture cancer health sciences |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725003132 |
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