Protocol for adeno-associated virus-mediated gene delivery to accelerate pancreatic carcinogenesis in mice
Summary: Introducing additional genetic modifications or environmental stimuli accelerates early pancreatic carcinogenesis in the p48Cre/+; LSL-KrasG12D/+ (KC) mouse model. Here, we present a protocol employing adeno-associated virus (AAV)-mediated gene delivery specifically in the pancreas to facil...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725003132 |
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| Summary: | Summary: Introducing additional genetic modifications or environmental stimuli accelerates early pancreatic carcinogenesis in the p48Cre/+; LSL-KrasG12D/+ (KC) mouse model. Here, we present a protocol employing adeno-associated virus (AAV)-mediated gene delivery specifically in the pancreas to facilitate the progression from acinar cells through acinar-to-ductal metaplasia (ADM) to pancreatic intraepithelial neoplasia (PanIN). We describe steps for AAV production, preparing KC mice, intra-pancreatic AAV injection, and analysis. This protocol allows the investigation of early events of pancreatic carcinogenesis in a spatially and temporally controlled manner.For complete details on the use and execution of this protocol, please refer to Li et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
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| ISSN: | 2666-1667 |