Integrated single-cell sequencing for the development of a GJA4-based precision immuno-prognostic model in melanoma
Methods: We conducted an analysis of RNA-seq and microarray data obtained from the TCGA and GEO databases, alongside single-cell RNA sequencing (scRNA-seq) data from glioma patients within the GEO repository. This comprehensive investigation, augmented by experimental studies, concentrated on explor...
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| Language: | English |
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Elsevier
2025-09-01
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| Series: | Translational Oncology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1936523325001810 |
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| author | Yantao Ding Si Xie Wenyang Nie Yun Bai Tianyu Yao Yixiao Wang Jiajie Chen Bo Liang Yi Zhou Hui Cheng Zaixing Wang Shengxiu Liu |
| author_facet | Yantao Ding Si Xie Wenyang Nie Yun Bai Tianyu Yao Yixiao Wang Jiajie Chen Bo Liang Yi Zhou Hui Cheng Zaixing Wang Shengxiu Liu |
| author_sort | Yantao Ding |
| collection | DOAJ |
| description | Methods: We conducted an analysis of RNA-seq and microarray data obtained from the TCGA and GEO databases, alongside single-cell RNA sequencing (scRNA-seq) data from glioma patients within the GEO repository. This comprehensive investigation, augmented by experimental studies, concentrated on exploring the interactions between tumor-associated endothelial cells (TECs) and tumors, as well as elucidating the molecular mechanisms involved. Results: Single-cell sequencing analysis identified differentially expressed genes within tumor-associated endothelial cells. Further investigation highlighted GJA4 as a pivotal marker gene for a terminal subpopulation, with its expression linked to poor prognosis. Subsequent experiments were conducted to explore its underlying functional mechanisms. Conclusions: GJA4 is highly expressed in melanoma patients, and its differential expression in tumor-associated endothelial cells influences melanoma proliferation and migration. GJA4-based risk models hold potential as predictive and therapeutic targets for personalized melanoma treatment. |
| format | Article |
| id | doaj-art-a4cb2dbd7a534ecbb4c4ba1c3b2d9feb |
| institution | DOAJ |
| issn | 1936-5233 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Translational Oncology |
| spelling | doaj-art-a4cb2dbd7a534ecbb4c4ba1c3b2d9feb2025-08-20T03:17:46ZengElsevierTranslational Oncology1936-52332025-09-015910245010.1016/j.tranon.2025.102450Integrated single-cell sequencing for the development of a GJA4-based precision immuno-prognostic model in melanomaYantao Ding0Si Xie1Wenyang Nie2Yun Bai3Tianyu Yao4Yixiao Wang5Jiajie Chen6Bo Liang7Yi Zhou8Hui Cheng9Zaixing Wang10Shengxiu Liu11Department of Dermatology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, China; Key Laboratory of Dermatology (Anhui Medical University), Ministry of Education, Hefei, Anhui 230022, China; Inflammation and Immune-Mediated Diseases Laboratory of Anhui Province, Hefei, Anhui 230022, ChinaDepartment of Dermatology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, China; Key Laboratory of Dermatology (Anhui Medical University), Ministry of Education, Hefei, Anhui 230022, China; Inflammation and Immune-Mediated Diseases Laboratory of Anhui Province, Hefei, Anhui 230022, ChinaFirst Clinical Medical College, Shandong University of Traditional Chinese Medicine, Jinan 250000, China; Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250000, ChinaDepartment of plastic surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, ChinaDepartment of Dermatology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, China; Key Laboratory of Dermatology (Anhui Medical University), Ministry of Education, Hefei, Anhui 230022, China; Inflammation and Immune-Mediated Diseases Laboratory of Anhui Province, Hefei, Anhui 230022, ChinaDepartment of Dermatology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, China; Key Laboratory of Dermatology (Anhui Medical University), Ministry of Education, Hefei, Anhui 230022, China; Inflammation and Immune-Mediated Diseases Laboratory of Anhui Province, Hefei, Anhui 230022, ChinaDepartment of Dermatology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, China; Key Laboratory of Dermatology (Anhui Medical University), Ministry of Education, Hefei, Anhui 230022, China; Inflammation and Immune-Mediated Diseases Laboratory of Anhui Province, Hefei, Anhui 230022, ChinaDepartment of Dermatology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, China; Key Laboratory of Dermatology (Anhui Medical University), Ministry of Education, Hefei, Anhui 230022, China; Inflammation and Immune-Mediated Diseases Laboratory of Anhui Province, Hefei, Anhui 230022, ChinaDepartment of Dermatology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, China; Key Laboratory of Dermatology (Anhui Medical University), Ministry of Education, Hefei, Anhui 230022, China; Inflammation and Immune-Mediated Diseases Laboratory of Anhui Province, Hefei, Anhui 230022, ChinaDepartment of Dermatology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, China; Key Laboratory of Dermatology (Anhui Medical University), Ministry of Education, Hefei, Anhui 230022, China; Inflammation and Immune-Mediated Diseases Laboratory of Anhui Province, Hefei, Anhui 230022, China; Corresponding authors at: Department of Dermatology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, China.Department of Dermatology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, China; Key Laboratory of Dermatology (Anhui Medical University), Ministry of Education, Hefei, Anhui 230022, China; Inflammation and Immune-Mediated Diseases Laboratory of Anhui Province, Hefei, Anhui 230022, China; Corresponding authors at: Department of Dermatology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, China.Department of Dermatology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, China; Key Laboratory of Dermatology (Anhui Medical University), Ministry of Education, Hefei, Anhui 230022, China; Inflammation and Immune-Mediated Diseases Laboratory of Anhui Province, Hefei, Anhui 230022, China; Corresponding authors at: Department of Dermatology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, China.Methods: We conducted an analysis of RNA-seq and microarray data obtained from the TCGA and GEO databases, alongside single-cell RNA sequencing (scRNA-seq) data from glioma patients within the GEO repository. This comprehensive investigation, augmented by experimental studies, concentrated on exploring the interactions between tumor-associated endothelial cells (TECs) and tumors, as well as elucidating the molecular mechanisms involved. Results: Single-cell sequencing analysis identified differentially expressed genes within tumor-associated endothelial cells. Further investigation highlighted GJA4 as a pivotal marker gene for a terminal subpopulation, with its expression linked to poor prognosis. Subsequent experiments were conducted to explore its underlying functional mechanisms. Conclusions: GJA4 is highly expressed in melanoma patients, and its differential expression in tumor-associated endothelial cells influences melanoma proliferation and migration. GJA4-based risk models hold potential as predictive and therapeutic targets for personalized melanoma treatment.http://www.sciencedirect.com/science/article/pii/S1936523325001810MelanomaGJA4DiagnosisPrognosisTumor-associated endothelial cellsPersonalized therapy |
| spellingShingle | Yantao Ding Si Xie Wenyang Nie Yun Bai Tianyu Yao Yixiao Wang Jiajie Chen Bo Liang Yi Zhou Hui Cheng Zaixing Wang Shengxiu Liu Integrated single-cell sequencing for the development of a GJA4-based precision immuno-prognostic model in melanoma Translational Oncology Melanoma GJA4 Diagnosis Prognosis Tumor-associated endothelial cells Personalized therapy |
| title | Integrated single-cell sequencing for the development of a GJA4-based precision immuno-prognostic model in melanoma |
| title_full | Integrated single-cell sequencing for the development of a GJA4-based precision immuno-prognostic model in melanoma |
| title_fullStr | Integrated single-cell sequencing for the development of a GJA4-based precision immuno-prognostic model in melanoma |
| title_full_unstemmed | Integrated single-cell sequencing for the development of a GJA4-based precision immuno-prognostic model in melanoma |
| title_short | Integrated single-cell sequencing for the development of a GJA4-based precision immuno-prognostic model in melanoma |
| title_sort | integrated single cell sequencing for the development of a gja4 based precision immuno prognostic model in melanoma |
| topic | Melanoma GJA4 Diagnosis Prognosis Tumor-associated endothelial cells Personalized therapy |
| url | http://www.sciencedirect.com/science/article/pii/S1936523325001810 |
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