ΦC31-mediated cassette exchange into a bacterial artificial chromosome
The use of bacterial artificial chromosomes (BACs) modified via homologous recombination in Escherichia coli has become a powerful tool in the transgenic field. Homologous recombination allows the manipulation of BACs in very different ways. However, this process can be cumbersome and problematic wh...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2007-11-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/000112592 |
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