Unveiling the role of long non-coding RNAs in chicken immune response to highly pathogenic avian influenza H5N1 infection
Avian influenza viruses (AIVs) pose a significant threat to global poultry production, necessitating effective control strategies to mitigate economic losses and ensure animal welfare. Long non-coding RNAs (lncRNAs) have emerged as crucial regulators of immune responses, yet their roles in AIV-infec...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124011027 |
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| author | Thi Hao Vu Chaeeun Kim Anh Duc Truong Jun-Mo Kim Hyun S. Lillehoj Yeong Ho Hong |
| author_facet | Thi Hao Vu Chaeeun Kim Anh Duc Truong Jun-Mo Kim Hyun S. Lillehoj Yeong Ho Hong |
| author_sort | Thi Hao Vu |
| collection | DOAJ |
| description | Avian influenza viruses (AIVs) pose a significant threat to global poultry production, necessitating effective control strategies to mitigate economic losses and ensure animal welfare. Long non-coding RNAs (lncRNAs) have emerged as crucial regulators of immune responses, yet their roles in AIV-infected chickens remain poorly understood. This study aimed to investigate the expression profiles of lncRNAs and their targets in Vietnamese Ri chickens infected with the highly pathogenic AIV (HPAIV) H5N1. Through RNA sequencing, we identified novel lncRNAs and analyzed differentially expressed (DE) transcripts at 1 and 3 days post-infection (dpi) in chicken lung tissue. Our results revealed a higher number of DE lncRNAs and mRNAs at 1 dpi and 3 dpi, respectively, compared to control, with resistant chickens exhibiting a notably stronger immune response than susceptible chickens at 3 dpi. Functional analysis implicated these lncRNAs in immune-related pathways crucial for host responses to H5N1 viral infection. Furthermore, we identified lncRNA–mRNA interactions associated with antiviral responses and immune function. Notably, several genes involved in antiviral resistance and immune responses showed higher expression in resistant chickens, confirming their stronger antiviral response. Overall, our study provides insights into the role of lncRNAs in the host's response to HPAIV H5N1 infection in chickens and highlights potential candidates for further investigation into host–pathogen interactions. These findings could drive the development of novel control strategies for AIVs, significantly enhancing poultry health and biosecurity. |
| format | Article |
| id | doaj-art-a42b072a35a64ece9a1c732cf3660563 |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-a42b072a35a64ece9a1c732cf36605632025-01-22T05:40:20ZengElsevierPoultry Science0032-57912025-01-011041104524Unveiling the role of long non-coding RNAs in chicken immune response to highly pathogenic avian influenza H5N1 infectionThi Hao Vu0Chaeeun Kim1Anh Duc Truong2Jun-Mo Kim3Hyun S. Lillehoj4Yeong Ho Hong5Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Republic of Korea; Department of Biochemistry and Immunology, National Institute of Veterinary Research, Hanoi 100000, VietnamDepartment of Animal Science and Technology, Chung-Ang University, Anseong 17546, Republic of KoreaDepartment of Biochemistry and Immunology, National Institute of Veterinary Research, Hanoi 100000, VietnamDepartment of Biochemistry and Immunology, National Institute of Veterinary Research, Hanoi 100000, VietnamAnimal Biosciences and Biotechnology Laboratory, Agricultural Research Services, United States Department of Agriculture, Beltsville 20705, MD, USADepartment of Animal Science and Technology, Chung-Ang University, Anseong 17546, Republic of Korea; Corresponding author.Avian influenza viruses (AIVs) pose a significant threat to global poultry production, necessitating effective control strategies to mitigate economic losses and ensure animal welfare. Long non-coding RNAs (lncRNAs) have emerged as crucial regulators of immune responses, yet their roles in AIV-infected chickens remain poorly understood. This study aimed to investigate the expression profiles of lncRNAs and their targets in Vietnamese Ri chickens infected with the highly pathogenic AIV (HPAIV) H5N1. Through RNA sequencing, we identified novel lncRNAs and analyzed differentially expressed (DE) transcripts at 1 and 3 days post-infection (dpi) in chicken lung tissue. Our results revealed a higher number of DE lncRNAs and mRNAs at 1 dpi and 3 dpi, respectively, compared to control, with resistant chickens exhibiting a notably stronger immune response than susceptible chickens at 3 dpi. Functional analysis implicated these lncRNAs in immune-related pathways crucial for host responses to H5N1 viral infection. Furthermore, we identified lncRNA–mRNA interactions associated with antiviral responses and immune function. Notably, several genes involved in antiviral resistance and immune responses showed higher expression in resistant chickens, confirming their stronger antiviral response. Overall, our study provides insights into the role of lncRNAs in the host's response to HPAIV H5N1 infection in chickens and highlights potential candidates for further investigation into host–pathogen interactions. These findings could drive the development of novel control strategies for AIVs, significantly enhancing poultry health and biosecurity.http://www.sciencedirect.com/science/article/pii/S0032579124011027Long non-coding RNAChickenLungAvian influenza virusH5N1 |
| spellingShingle | Thi Hao Vu Chaeeun Kim Anh Duc Truong Jun-Mo Kim Hyun S. Lillehoj Yeong Ho Hong Unveiling the role of long non-coding RNAs in chicken immune response to highly pathogenic avian influenza H5N1 infection Poultry Science Long non-coding RNA Chicken Lung Avian influenza virus H5N1 |
| title | Unveiling the role of long non-coding RNAs in chicken immune response to highly pathogenic avian influenza H5N1 infection |
| title_full | Unveiling the role of long non-coding RNAs in chicken immune response to highly pathogenic avian influenza H5N1 infection |
| title_fullStr | Unveiling the role of long non-coding RNAs in chicken immune response to highly pathogenic avian influenza H5N1 infection |
| title_full_unstemmed | Unveiling the role of long non-coding RNAs in chicken immune response to highly pathogenic avian influenza H5N1 infection |
| title_short | Unveiling the role of long non-coding RNAs in chicken immune response to highly pathogenic avian influenza H5N1 infection |
| title_sort | unveiling the role of long non coding rnas in chicken immune response to highly pathogenic avian influenza h5n1 infection |
| topic | Long non-coding RNA Chicken Lung Avian influenza virus H5N1 |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124011027 |
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