Guidelines for optimized gene knockout using CRISPR/Cas9

Guidelines for optimized gene knockout using CRISPR/Cas9

CRISPR/Cas9 technology has evolved as the most powerful approach to generate genetic models both for fundamental and preclinical research. Despite its apparent simplicity, the outcome of a genome-editing experiment can be substantially impacted by technical parameters and biological considerations....

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Bibliographic Details
Main Authors: Claude Van Campenhout, Pauline Cabochette, Anne-Clémence Veillard, Miklos Laczik, Agnieszka Zelisko-Schmidt, Céline Sabatel, Maxime Dhainaut, Benoit Vanhollebeke, Cyril Gueydan, Véronique Kruys
Format: Article
Language:English
Published: Taylor & Francis Group 2019-06-01
Series:BioTechniques
Subjects:
gene targeting
methodology
quality control
Online Access:https://www.future-science.com/doi/10.2144/btn-2018-0187
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https://www.future-science.com/doi/10.2144/btn-2018-0187

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