Screening and characterization of integration sites based on CRISPR-Cpf1 in Pichia pastoris
Pichia pastoris, a methylotrophic yeast, can utilize methanol as a carbon source and energy source to synthesize high-value chemicals, and is an ideal host for biomanufacturing. Constructing the P. pastoris cell factory is somewhat impeded due to the absence of genetic tools for manipulating multi-g...
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Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
KeAi Communications Co., Ltd.
2024-12-01
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Series: | Synthetic and Systems Biotechnology |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2405805X24000917 |
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