Rapid and high-throughput screening of proteolysis targeting chimeras using a dual-reporter system expressing fluorescence protein and luciferase

Rapid and high-throughput screening of proteolysis targeting chimeras using a dual-reporter system expressing fluorescence protein and luciferase

Abstract Background Proteolysis targeting chimera (PROTAC), a novel drug discovery strategy, utilizes the ubiquitin–proteasome system to degrade target proteins in cells. While Western blotting, mass spectrometry, and Lumit Immunoassay have been instrumental in determining protein levels, the rapid...

Full description

Saved in:
Bibliographic Details
Main Authors: Shuai Meng, Yuan Meng, Xuena Yang, Wenwen Yu, Bole Li, Tianjun Liu, Jie Zhang, Xiubao Ren, Lin Zhang
Format: Article
Language:English
Published: BMC 2025-02-01
Series:BMC Biology
Subjects:
Proteolysis targeting chimera
High-throughput screening
Fluorescence
Luciferase
Sirtuin 2
Online Access:https://doi.org/10.1186/s12915-025-02153-7
Tags: Add Tag
No Tags, Be the first to tag this record!

Internet

https://doi.org/10.1186/s12915-025-02153-7

Similar Items