Characteristics of Bacterial Community Structure in Yellow Paddy Soil After Long-Term Chemical Fertilisation, Organic Fertilisation, and Fertilisation Mode Conversion

This study aimed to compare bacterial community structure differences in yellow paddy soil under long-term chemical/organic fertilisation and fertiliser conversion to guide farmland fertilisation strategies in yellow loam areas. Treatments included (1) continuous application of chemical fertilisers...

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Main Authors: Yehua Yang, Xingcheng Huang, Huaqing Zhu, Yanling Liu, Yarong Zhang, Song Zhang, Han Xiong, Huan Yang, Yu Li
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Agronomy
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Online Access:https://www.mdpi.com/2073-4395/15/3/749
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author Yehua Yang
Xingcheng Huang
Huaqing Zhu
Yanling Liu
Yarong Zhang
Song Zhang
Han Xiong
Huan Yang
Yu Li
author_facet Yehua Yang
Xingcheng Huang
Huaqing Zhu
Yanling Liu
Yarong Zhang
Song Zhang
Han Xiong
Huan Yang
Yu Li
author_sort Yehua Yang
collection DOAJ
description This study aimed to compare bacterial community structure differences in yellow paddy soil under long-term chemical/organic fertilisation and fertiliser conversion to guide farmland fertilisation strategies in yellow loam areas. Treatments included (1) continuous application of chemical fertilisers for 27 years (CF-CF); (2) application of chemical fertiliser continuously for 24 years and then application of organic fertiliser for 3 years (CF-OF); (3) continuous application of organic fertiliser for 27 years (OF-OF); and (4) application of organic fertiliser continuously for 24 years and then application of chemical fertiliser for 3 years (OF-CF). The results show that long-term fertilisation alters genus-level bacterial taxa, while fertilisation mode changes significantly increase taxa quantities at both phylum and genus levels. Different fertilisation treatments affect the relative abundance of bacteria; the relative abundance of <i>Firmicutes</i> in OF-OF is significantly greater than that in CF-CF, while <i>Gemmatimonadota</i> and <i>Patescibacteria</i> show the opposite trend. Compared to CF-CF, CF-OF increases the relative abundance of <i>Firmicutes</i> and decreases that of <i>Cyanobacteria</i>, whereas OF-CF increases the relative abundance of <i>Firmicutes</i> compared to OF-OF. Notably, <i>Patescibacteria</i> is significantly enriched in CF-CF, while <i>Cyanobacteria</i> and <i>Bacteroidota</i> are significantly enriched in CF-OF, and <i>Firmicutes</i> and <i>Myxomycophyta</i> are significantly enriched in the OF-OF treatment. The bacterial community composition of CF-CF and CF-OF is similar, while the bacterial community composition of OF-OF and OF-CF is similar. In bacterial assembly processes, OF-CF improves the heterogeneous selection process and reduces the homogeneous dispersal process compared to OF-OF. The bacterial assembly process of OF-CF gradually becomes similar to that of CF-CF and CF-OF. Further analyses indicate that fertilisation influences the soil bacterial community composition by affecting total nitrogen, organic matter, available phosphorus, and pH. Overall, long-term different fertilisation predominates bacterial community distribution, while short-term changes in fertilisation mode have a smaller but significant effect on bacterial community distribution, influencing the quantity and relative abundance of bacterial taxa; the application of organic fertilisers is more beneficial for the even distribution of bacteria.
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series Agronomy
spelling doaj-art-a133100f50e74addbe7722944fd39fdf2025-08-20T02:11:04ZengMDPI AGAgronomy2073-43952025-03-0115374910.3390/agronomy15030749Characteristics of Bacterial Community Structure in Yellow Paddy Soil After Long-Term Chemical Fertilisation, Organic Fertilisation, and Fertilisation Mode ConversionYehua Yang0Xingcheng Huang1Huaqing Zhu2Yanling Liu3Yarong Zhang4Song Zhang5Han Xiong6Huan Yang7Yu Li8Institute of Soil and Fertilizer, Guizhou Academy of Agricultural Sciences, Guiyang 550006, ChinaInstitute of Soil and Fertilizer, Guizhou Academy of Agricultural Sciences, Guiyang 550006, ChinaInstitute of Soil and Fertilizer, Guizhou Academy of Agricultural Sciences, Guiyang 550006, ChinaInstitute of Soil and Fertilizer, Guizhou Academy of Agricultural Sciences, Guiyang 550006, ChinaInstitute of Soil and Fertilizer, Guizhou Academy of Agricultural Sciences, Guiyang 550006, ChinaGuiding Agricultural and Rural Bureau, Guiding 551300, ChinaInstitute of Soil and Fertilizer, Guizhou Academy of Agricultural Sciences, Guiyang 550006, ChinaInstitute of Soil and Fertilizer, Guizhou Academy of Agricultural Sciences, Guiyang 550006, ChinaInstitute of Soil and Fertilizer, Guizhou Academy of Agricultural Sciences, Guiyang 550006, ChinaThis study aimed to compare bacterial community structure differences in yellow paddy soil under long-term chemical/organic fertilisation and fertiliser conversion to guide farmland fertilisation strategies in yellow loam areas. Treatments included (1) continuous application of chemical fertilisers for 27 years (CF-CF); (2) application of chemical fertiliser continuously for 24 years and then application of organic fertiliser for 3 years (CF-OF); (3) continuous application of organic fertiliser for 27 years (OF-OF); and (4) application of organic fertiliser continuously for 24 years and then application of chemical fertiliser for 3 years (OF-CF). The results show that long-term fertilisation alters genus-level bacterial taxa, while fertilisation mode changes significantly increase taxa quantities at both phylum and genus levels. Different fertilisation treatments affect the relative abundance of bacteria; the relative abundance of <i>Firmicutes</i> in OF-OF is significantly greater than that in CF-CF, while <i>Gemmatimonadota</i> and <i>Patescibacteria</i> show the opposite trend. Compared to CF-CF, CF-OF increases the relative abundance of <i>Firmicutes</i> and decreases that of <i>Cyanobacteria</i>, whereas OF-CF increases the relative abundance of <i>Firmicutes</i> compared to OF-OF. Notably, <i>Patescibacteria</i> is significantly enriched in CF-CF, while <i>Cyanobacteria</i> and <i>Bacteroidota</i> are significantly enriched in CF-OF, and <i>Firmicutes</i> and <i>Myxomycophyta</i> are significantly enriched in the OF-OF treatment. The bacterial community composition of CF-CF and CF-OF is similar, while the bacterial community composition of OF-OF and OF-CF is similar. In bacterial assembly processes, OF-CF improves the heterogeneous selection process and reduces the homogeneous dispersal process compared to OF-OF. The bacterial assembly process of OF-CF gradually becomes similar to that of CF-CF and CF-OF. Further analyses indicate that fertilisation influences the soil bacterial community composition by affecting total nitrogen, organic matter, available phosphorus, and pH. Overall, long-term different fertilisation predominates bacterial community distribution, while short-term changes in fertilisation mode have a smaller but significant effect on bacterial community distribution, influencing the quantity and relative abundance of bacterial taxa; the application of organic fertilisers is more beneficial for the even distribution of bacteria.https://www.mdpi.com/2073-4395/15/3/749yellow paddy soilchemical fertiliserorganic fertiliserbacteriainfluencing factors
spellingShingle Yehua Yang
Xingcheng Huang
Huaqing Zhu
Yanling Liu
Yarong Zhang
Song Zhang
Han Xiong
Huan Yang
Yu Li
Characteristics of Bacterial Community Structure in Yellow Paddy Soil After Long-Term Chemical Fertilisation, Organic Fertilisation, and Fertilisation Mode Conversion
Agronomy
yellow paddy soil
chemical fertiliser
organic fertiliser
bacteria
influencing factors
title Characteristics of Bacterial Community Structure in Yellow Paddy Soil After Long-Term Chemical Fertilisation, Organic Fertilisation, and Fertilisation Mode Conversion
title_full Characteristics of Bacterial Community Structure in Yellow Paddy Soil After Long-Term Chemical Fertilisation, Organic Fertilisation, and Fertilisation Mode Conversion
title_fullStr Characteristics of Bacterial Community Structure in Yellow Paddy Soil After Long-Term Chemical Fertilisation, Organic Fertilisation, and Fertilisation Mode Conversion
title_full_unstemmed Characteristics of Bacterial Community Structure in Yellow Paddy Soil After Long-Term Chemical Fertilisation, Organic Fertilisation, and Fertilisation Mode Conversion
title_short Characteristics of Bacterial Community Structure in Yellow Paddy Soil After Long-Term Chemical Fertilisation, Organic Fertilisation, and Fertilisation Mode Conversion
title_sort characteristics of bacterial community structure in yellow paddy soil after long term chemical fertilisation organic fertilisation and fertilisation mode conversion
topic yellow paddy soil
chemical fertiliser
organic fertiliser
bacteria
influencing factors
url https://www.mdpi.com/2073-4395/15/3/749
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