High-fiber basil seed flour reduces insulin resistance and hepatic steatosis in high-fat diet mice

Abstract The incidence of insulin resistance (IR) and hepatic steatosis is increasing, with dietary fiber playing a protective role against these disorders. Ocimum basilicum L., widely used in food, pharmaceutical, and cosmetic industries, but their health-promoting properties remain underexplored....

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Main Authors: Camila Farías, Camila Cisternas, Angie Caicedo, Lorena Mercado, Rodrigo Valenzuela, Héctor Calderón, Alejandra Espinosa, L. A. Videla, Loreto A. Muñoz
Format: Article
Language:English
Published: Nature Portfolio 2024-11-01
Series:npj Science of Food
Online Access:https://doi.org/10.1038/s41538-024-00329-z
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author Camila Farías
Camila Cisternas
Angie Caicedo
Lorena Mercado
Rodrigo Valenzuela
Héctor Calderón
Alejandra Espinosa
L. A. Videla
Loreto A. Muñoz
author_facet Camila Farías
Camila Cisternas
Angie Caicedo
Lorena Mercado
Rodrigo Valenzuela
Héctor Calderón
Alejandra Espinosa
L. A. Videla
Loreto A. Muñoz
author_sort Camila Farías
collection DOAJ
description Abstract The incidence of insulin resistance (IR) and hepatic steatosis is increasing, with dietary fiber playing a protective role against these disorders. Ocimum basilicum L., widely used in food, pharmaceutical, and cosmetic industries, but their health-promoting properties remain underexplored. This study evaluated the effects of a fiber-rich fraction of partially defatted basil seeds (BSF) on IR, hepatic steatosis, and polyunsaturated fatty acid and short-chain fatty acid (SCFA) profiles in high-fat diet (HFD)-fed C57BL/6 J male mice. Mice were assigned to four groups and fed either a control diet or HFD, supplemented with BSF or oat flour for 4 weeks. HFD induced IR, hepatic steatosis, proinflammatory state, and a significant decreased in SCFA production. In contrast, supplementation with BSF attenuated IR, steatosis, liver damage, oxidative stress, and inflammation, while increasing n-3 polyunsaturated fatty acids in liver, adipocytes, and erythrocytes, and enhancing SCFA production, suggesting potential therapeutic benefits in managing these conditions.
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language English
publishDate 2024-11-01
publisher Nature Portfolio
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series npj Science of Food
spelling doaj-art-a0f3d6257773401dbdbe20e8a3d420222025-08-20T02:13:55ZengNature Portfolionpj Science of Food2396-83702024-11-018111510.1038/s41538-024-00329-zHigh-fiber basil seed flour reduces insulin resistance and hepatic steatosis in high-fat diet miceCamila Farías0Camila Cisternas1Angie Caicedo2Lorena Mercado3Rodrigo Valenzuela4Héctor Calderón5Alejandra Espinosa6L. A. Videla7Loreto A. Muñoz8Nutrition Department, Faculty of Medicine, University of ChileSchool of Health Care Sciences , Universidad San SebastiánSchool of Agronomy, Faculty of Agronomy and Food Sciences , Pontificia Universidad Católica de ValparaísoNutrition Department, Faculty of Medicine, University of ChileNutrition Department, Faculty of Medicine, University of ChileFood Science Lab, Faculty of Medicine and Health Sciences, Universidad Central de ChileCenter of Interdisciplinary Biomedical and Engineering Research for Health-MEDING. Universidad de ValparaísoMolecular and Clinical Pharmacology Program, Institute of Biomedical Sciences, Faculty of Medicine, Universidad de ChileFood Science Lab, Faculty of Medicine and Health Sciences, Universidad Central de ChileAbstract The incidence of insulin resistance (IR) and hepatic steatosis is increasing, with dietary fiber playing a protective role against these disorders. Ocimum basilicum L., widely used in food, pharmaceutical, and cosmetic industries, but their health-promoting properties remain underexplored. This study evaluated the effects of a fiber-rich fraction of partially defatted basil seeds (BSF) on IR, hepatic steatosis, and polyunsaturated fatty acid and short-chain fatty acid (SCFA) profiles in high-fat diet (HFD)-fed C57BL/6 J male mice. Mice were assigned to four groups and fed either a control diet or HFD, supplemented with BSF or oat flour for 4 weeks. HFD induced IR, hepatic steatosis, proinflammatory state, and a significant decreased in SCFA production. In contrast, supplementation with BSF attenuated IR, steatosis, liver damage, oxidative stress, and inflammation, while increasing n-3 polyunsaturated fatty acids in liver, adipocytes, and erythrocytes, and enhancing SCFA production, suggesting potential therapeutic benefits in managing these conditions.https://doi.org/10.1038/s41538-024-00329-z
spellingShingle Camila Farías
Camila Cisternas
Angie Caicedo
Lorena Mercado
Rodrigo Valenzuela
Héctor Calderón
Alejandra Espinosa
L. A. Videla
Loreto A. Muñoz
High-fiber basil seed flour reduces insulin resistance and hepatic steatosis in high-fat diet mice
npj Science of Food
title High-fiber basil seed flour reduces insulin resistance and hepatic steatosis in high-fat diet mice
title_full High-fiber basil seed flour reduces insulin resistance and hepatic steatosis in high-fat diet mice
title_fullStr High-fiber basil seed flour reduces insulin resistance and hepatic steatosis in high-fat diet mice
title_full_unstemmed High-fiber basil seed flour reduces insulin resistance and hepatic steatosis in high-fat diet mice
title_short High-fiber basil seed flour reduces insulin resistance and hepatic steatosis in high-fat diet mice
title_sort high fiber basil seed flour reduces insulin resistance and hepatic steatosis in high fat diet mice
url https://doi.org/10.1038/s41538-024-00329-z
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