Monitoring of Nutrients, Metabolites, IgG Titer, and Cell Densities in 10 L Bioreactors Using Raman Spectroscopy and PLS Regression Models

Monitoring of Nutrients, Metabolites, IgG Titer, and Cell Densities in 10 L Bioreactors Using Raman Spectroscopy and PLS Regression Models

<b>Background:</b> Chinese hamster ovary (CHO) cell metabolism is complex, influenced by nutrients like glucose and glutamine and metabolites such as lactate. Real-time monitoring is necessary for optimizing culture conditions and ensuring consistent product quality. Raman spectroscopy h...

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Main Authors: Morandise Rubini, Julien Boyer, Jordane Poulain, Anaïs Berger, Thomas Saillard, Julien Louet, Martin Soucé, Sylvie Roussel, Sylvain Arnould, Murielle Vergès, Fabien Chauchard-Rios, Igor Chourpa
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Pharmaceutics
Subjects:
Raman spectroscopy
process analytical technology (PAT)
CHO cell culture
bioprocess monitoring
chemometric analysis
IgG titer prediction
Online Access:https://www.mdpi.com/1999-4923/17/4/473
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