The Influence of Vitamin C–incorporated Polycaprolactone on Osteogenesis in Osteoblast-Osteoclast Co-culture In Vitro
ABSTRACT: Objectives: Implantation of biomaterials generates reactive oxygen species (ROS) in the peri-implant microenvironment. Bone loss occurs when ROS levels exceed the local antioxidant capacity. The aim of this study was to investigate the influence of vitamin C–incorporated polycaprolactone...
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Elsevier
2025-10-01
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| Series: | International Dental Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0020653925002369 |
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| author | Elaf Akram Abdulhameed K.G. Aghila Rani Ensanya A. Abou Neel Nadia Khalifa Yanti Johari Marzuki Omar Ab Rani Samsudin |
| author_facet | Elaf Akram Abdulhameed K.G. Aghila Rani Ensanya A. Abou Neel Nadia Khalifa Yanti Johari Marzuki Omar Ab Rani Samsudin |
| author_sort | Elaf Akram Abdulhameed |
| collection | DOAJ |
| description | ABSTRACT: Objectives: Implantation of biomaterials generates reactive oxygen species (ROS) in the peri-implant microenvironment. Bone loss occurs when ROS levels exceed the local antioxidant capacity. The aim of this study was to investigate the influence of vitamin C–incorporated polycaprolactone (PCL-Vit C) membrane in scavenging ROS and enhancing biomineralisation in osteoblast–osteoclast (OB-OC) co-culture system. Methods: OB-OCs were cultured on polycaprolactone (PCL) and PCL-Vit C membranes under osteogenic conditions to mimic the bone microenvironment. ROS generation was measured using flow cytometry. ALP and the RANKL/OPG ratio were determined by colorimetric and ELISA assays, respectively. Gene expression of ALP, Col1, Runx-2 and OCN and protein expression of Runx-2, BMP-7, Col1 and OCN were determined by real-time PCR and western blotting, respectively. Activation of P38, ERK and JNK and beta-catenin expression was also analysed. Results: OB-OC grown on PCL membrane generated a higher amount of ROS compared to those on PCL-Vit C. Colorimetric assays revealed a significantly higher ALP activity in OB-OC co-cultures on PCL-Vit C membranes. Furthermore, OB-OC grown on PCL-Vit C membrane showed significant upregulation in mRNA levels of ALP, Col1 and OCN with lower RANKL/OPG ratio and higher amounts of mineralisation nodules. Runx-2 expression was comparable in both membranes. Western blotting showed a significant increase in phosphorylation of P38 MAPK, ERK, JNK and beta-catenin expression in OB-OC maintained on PCL membrane, plausibly due to increased ROS levels, compared to PCL-Vit C. Significance: OB-OC grown on PCL-Vit C membrane scavenged ROS and supported a higher osteogenic potential environment for OB-OC co-culture in vitro. |
| format | Article |
| id | doaj-art-a05fd0d6399446f7beecc2092e420183 |
| institution | Kabale University |
| issn | 0020-6539 |
| language | English |
| publishDate | 2025-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Dental Journal |
| spelling | doaj-art-a05fd0d6399446f7beecc2092e4201832025-08-22T04:54:39ZengElsevierInternational Dental Journal0020-65392025-10-0175510094910.1016/j.identj.2025.100949The Influence of Vitamin C–incorporated Polycaprolactone on Osteogenesis in Osteoblast-Osteoclast Co-culture In VitroElaf Akram Abdulhameed0K.G. Aghila Rani1Ensanya A. Abou Neel2Nadia Khalifa3Yanti Johari4Marzuki Omar5Ab Rani Samsudin6Restorative & Preventive Dentistry Department, College of Dental Medicine, University of Sharjah, Sharjah, United Arab Emirates; School of Dental Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, MalaysiaResearch Institute for Medical and Health Sciences, University of Sharjah, Sharjah, United Arab EmiratesRestorative & Preventive Dentistry Department, College of Dental Medicine, University of Sharjah, Sharjah, United Arab Emirates; UCL Eastman Dental Institute, Biomaterials & Tissue Engineering Division, Royal Free Hospital, Rowland Hill Street, London, UKRestorative & Preventive Dentistry Department, College of Dental Medicine, University of Sharjah, Sharjah, United Arab EmiratesSchool of Dental Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, MalaysiaSchool of Dental Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia; Corresponding authors. College of Dental Medicine, Oral & Craniofacial Health Sciences; School of Dental Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia, Tel: +97165057600.Oral & Craniofacial Health Sciences, College of Dental Medicine, University of Sharjah, Sharjah, United Arab Emirates; Corresponding authors. College of Dental Medicine, Oral & Craniofacial Health Sciences; School of Dental Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia, Tel: +97165057600.ABSTRACT: Objectives: Implantation of biomaterials generates reactive oxygen species (ROS) in the peri-implant microenvironment. Bone loss occurs when ROS levels exceed the local antioxidant capacity. The aim of this study was to investigate the influence of vitamin C–incorporated polycaprolactone (PCL-Vit C) membrane in scavenging ROS and enhancing biomineralisation in osteoblast–osteoclast (OB-OC) co-culture system. Methods: OB-OCs were cultured on polycaprolactone (PCL) and PCL-Vit C membranes under osteogenic conditions to mimic the bone microenvironment. ROS generation was measured using flow cytometry. ALP and the RANKL/OPG ratio were determined by colorimetric and ELISA assays, respectively. Gene expression of ALP, Col1, Runx-2 and OCN and protein expression of Runx-2, BMP-7, Col1 and OCN were determined by real-time PCR and western blotting, respectively. Activation of P38, ERK and JNK and beta-catenin expression was also analysed. Results: OB-OC grown on PCL membrane generated a higher amount of ROS compared to those on PCL-Vit C. Colorimetric assays revealed a significantly higher ALP activity in OB-OC co-cultures on PCL-Vit C membranes. Furthermore, OB-OC grown on PCL-Vit C membrane showed significant upregulation in mRNA levels of ALP, Col1 and OCN with lower RANKL/OPG ratio and higher amounts of mineralisation nodules. Runx-2 expression was comparable in both membranes. Western blotting showed a significant increase in phosphorylation of P38 MAPK, ERK, JNK and beta-catenin expression in OB-OC maintained on PCL membrane, plausibly due to increased ROS levels, compared to PCL-Vit C. Significance: OB-OC grown on PCL-Vit C membrane scavenged ROS and supported a higher osteogenic potential environment for OB-OC co-culture in vitro.http://www.sciencedirect.com/science/article/pii/S0020653925002369Osteoblast–osteoclast co-culturePolycaprolactoneVitamin CReactive oxygen speciesOxidative stressOsteogenic potential |
| spellingShingle | Elaf Akram Abdulhameed K.G. Aghila Rani Ensanya A. Abou Neel Nadia Khalifa Yanti Johari Marzuki Omar Ab Rani Samsudin The Influence of Vitamin C–incorporated Polycaprolactone on Osteogenesis in Osteoblast-Osteoclast Co-culture In Vitro International Dental Journal Osteoblast–osteoclast co-culture Polycaprolactone Vitamin C Reactive oxygen species Oxidative stress Osteogenic potential |
| title | The Influence of Vitamin C–incorporated Polycaprolactone on Osteogenesis in Osteoblast-Osteoclast Co-culture In Vitro |
| title_full | The Influence of Vitamin C–incorporated Polycaprolactone on Osteogenesis in Osteoblast-Osteoclast Co-culture In Vitro |
| title_fullStr | The Influence of Vitamin C–incorporated Polycaprolactone on Osteogenesis in Osteoblast-Osteoclast Co-culture In Vitro |
| title_full_unstemmed | The Influence of Vitamin C–incorporated Polycaprolactone on Osteogenesis in Osteoblast-Osteoclast Co-culture In Vitro |
| title_short | The Influence of Vitamin C–incorporated Polycaprolactone on Osteogenesis in Osteoblast-Osteoclast Co-culture In Vitro |
| title_sort | influence of vitamin c incorporated polycaprolactone on osteogenesis in osteoblast osteoclast co culture in vitro |
| topic | Osteoblast–osteoclast co-culture Polycaprolactone Vitamin C Reactive oxygen species Oxidative stress Osteogenic potential |
| url | http://www.sciencedirect.com/science/article/pii/S0020653925002369 |
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