Serum Inflammatory Cytokines and Growth Factors in Patients with Sjögren’s Syndrome and Diabetes Mellitus
Abstract Background/ Aims: Sjögren’s syndrome (SS) and diabetes mellitus (DM) are common causes of dry eye disease (DED) and autologous serum is used when artificial tears are not sufficient. Our aim was to compare serum growth factor and inflammatory cytokine levels of SS and DM patients with the h...
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Selcuk University Press
2023-08-01
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| Series: | Genel Tıp Dergisi |
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| Online Access: | https://dergipark.org.tr/tr/download/article-file/3208484 |
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| author | Yalçın Karaküçük Sema Yilmaz Ali Unlu Banu Bozkurt Şule Nur Acar Duyan |
| author_facet | Yalçın Karaküçük Sema Yilmaz Ali Unlu Banu Bozkurt Şule Nur Acar Duyan |
| author_sort | Yalçın Karaküçük |
| collection | DOAJ |
| description | Abstract
Background/ Aims: Sjögren’s syndrome (SS) and diabetes mellitus (DM) are common causes of dry eye disease (DED) and autologous serum is used when artificial tears are not sufficient. Our aim was to compare serum growth factor and inflammatory cytokine levels of SS and DM patients with the healthy individuals.
Methods: Twenty-three SS patients (mean age 52.70±7.55 years), 25 DM patients (mean age 56.68±6.53 years), and 23 healthy subjects (mean age 51.70±9.14 years) were included in the study. After detailed ophthalmological examination, Schirmer test, tear break-up time (TBUT) and Ocular Surface Disease Index (OSDI) scores were measured. Serum levels of six different proinflammatory interleukins (IL), five growth factors, matrix metalloproteinase-9, and fibronectin were measured by immunoassay. One-way ANOVA or Kruskal-Wallis tests and Dunn-Bonferroni post hoc analysis were used for comparison and p<0.05 was considered significant.
Results: Schirmer test and TBUT were significantly lower in the SS group (2.08±1.72 mm/5 min and 3.08±2.08 s) than in the DR (10.24±4.63 mm/5 min and 4.20±3.09 s) and control groups (13.30±5.95 mm/5 min and 9.00±1.75 s) (p<0.001). Among the parameters studied, mean serum IL-23 level was significantly higher in the SS group (156.66±207.94 pg/mL) than in the DM and control groups (73.48±95.91 and 69.59±105.39 pg/mL, respectively) (p<0.05). Serum insulin-like growth factor 1 (IGF-1) level was lowest in DM patients (DM: 12.89±21.09, SS: 30.77±19.85, and control: 27.08±21.93 ng/mL) (p<0.05). Sjögren’s syndrome disease activity index (ESSDAI) showed a negative correlation with TBUT and a positive correlation with IL-1, IL-2 and fibronectin (p<0.005).
Conclusions: Except IL-23 and IGF-1, the contents of serum obtained from patients with SS and DM are similar with the healthy individuals. Therefore, autologous serum seems to be a good option to replace deficient tear fluid in these subjects. |
| format | Article |
| id | doaj-art-9fe1e512d84446c88838d9a7696f3e15 |
| institution | Kabale University |
| issn | 2602-3741 |
| language | English |
| publishDate | 2023-08-01 |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Genel Tıp Dergisi |
| spelling | doaj-art-9fe1e512d84446c88838d9a7696f3e152025-01-03T01:05:22ZengSelcuk University PressGenel Tıp Dergisi2602-37412023-08-0133436036610.54005/geneltip.1314282154Serum Inflammatory Cytokines and Growth Factors in Patients with Sjögren’s Syndrome and Diabetes MellitusYalçın Karaküçük0Sema Yilmaz1Ali Unlu2Banu Bozkurt3Şule Nur Acar Duyan4Kudret Göz HastanesiSELÇUK ÜNİVERSİTESİ, TIP FAKÜLTESİSELÇUK ÜNİVERSİTESİ, TIP FAKÜLTESİSELÇUK ÜNİVERSİTESİ, TIP FAKÜLTESİKonya Şehir HastanesiAbstract Background/ Aims: Sjögren’s syndrome (SS) and diabetes mellitus (DM) are common causes of dry eye disease (DED) and autologous serum is used when artificial tears are not sufficient. Our aim was to compare serum growth factor and inflammatory cytokine levels of SS and DM patients with the healthy individuals. Methods: Twenty-three SS patients (mean age 52.70±7.55 years), 25 DM patients (mean age 56.68±6.53 years), and 23 healthy subjects (mean age 51.70±9.14 years) were included in the study. After detailed ophthalmological examination, Schirmer test, tear break-up time (TBUT) and Ocular Surface Disease Index (OSDI) scores were measured. Serum levels of six different proinflammatory interleukins (IL), five growth factors, matrix metalloproteinase-9, and fibronectin were measured by immunoassay. One-way ANOVA or Kruskal-Wallis tests and Dunn-Bonferroni post hoc analysis were used for comparison and p<0.05 was considered significant. Results: Schirmer test and TBUT were significantly lower in the SS group (2.08±1.72 mm/5 min and 3.08±2.08 s) than in the DR (10.24±4.63 mm/5 min and 4.20±3.09 s) and control groups (13.30±5.95 mm/5 min and 9.00±1.75 s) (p<0.001). Among the parameters studied, mean serum IL-23 level was significantly higher in the SS group (156.66±207.94 pg/mL) than in the DM and control groups (73.48±95.91 and 69.59±105.39 pg/mL, respectively) (p<0.05). Serum insulin-like growth factor 1 (IGF-1) level was lowest in DM patients (DM: 12.89±21.09, SS: 30.77±19.85, and control: 27.08±21.93 ng/mL) (p<0.05). Sjögren’s syndrome disease activity index (ESSDAI) showed a negative correlation with TBUT and a positive correlation with IL-1, IL-2 and fibronectin (p<0.005). Conclusions: Except IL-23 and IGF-1, the contents of serum obtained from patients with SS and DM are similar with the healthy individuals. Therefore, autologous serum seems to be a good option to replace deficient tear fluid in these subjects.https://dergipark.org.tr/tr/download/article-file/3208484i̇nflamatuar sitokinlerbüyüme faktörlerisjögren sendromudiabetes mellitusotolog seruminflammatory cytokinesgrowth factorssjögren’s syndromediabetic mellitusautologous serum |
| spellingShingle | Yalçın Karaküçük Sema Yilmaz Ali Unlu Banu Bozkurt Şule Nur Acar Duyan Serum Inflammatory Cytokines and Growth Factors in Patients with Sjögren’s Syndrome and Diabetes Mellitus Genel Tıp Dergisi i̇nflamatuar sitokinler büyüme faktörleri sjögren sendromu diabetes mellitus otolog serum inflammatory cytokines growth factors sjögren’s syndrome diabetic mellitus autologous serum |
| title | Serum Inflammatory Cytokines and Growth Factors in Patients with Sjögren’s Syndrome and Diabetes Mellitus |
| title_full | Serum Inflammatory Cytokines and Growth Factors in Patients with Sjögren’s Syndrome and Diabetes Mellitus |
| title_fullStr | Serum Inflammatory Cytokines and Growth Factors in Patients with Sjögren’s Syndrome and Diabetes Mellitus |
| title_full_unstemmed | Serum Inflammatory Cytokines and Growth Factors in Patients with Sjögren’s Syndrome and Diabetes Mellitus |
| title_short | Serum Inflammatory Cytokines and Growth Factors in Patients with Sjögren’s Syndrome and Diabetes Mellitus |
| title_sort | serum inflammatory cytokines and growth factors in patients with sjogren s syndrome and diabetes mellitus |
| topic | i̇nflamatuar sitokinler büyüme faktörleri sjögren sendromu diabetes mellitus otolog serum inflammatory cytokines growth factors sjögren’s syndrome diabetic mellitus autologous serum |
| url | https://dergipark.org.tr/tr/download/article-file/3208484 |
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