Protocol for the imputation of stimulus-induced single-cell gene expression trajectories from time-series scRNA-seq data

Protocol for the imputation of stimulus-induced single-cell gene expression trajectories from time-series scRNA-seq data

Summary: Single-cell RNA sequencing (scRNA-seq) measures cell-to-cell heterogeneous mRNA abundance but destroys the cell and precludes tracking of heterogeneous gene expression trajectories. Here, we present an approach to impute single-cell gene expression trajectories (scGETs) from time-series scR...

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Bibliographic Details
Main Authors: Katherine M. Sheu, Alexander Hoffmann
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:STAR Protocols
Subjects:
bioinformatics
RNA-seq
molecular biology
gene expression
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725002175
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http://www.sciencedirect.com/science/article/pii/S2666166725002175

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