Metabolomic Insights into the Antimicrobial Effects of <i>Metschnikowia</i> Yeast on Phytopathogens
One of the most important features of <i>Metschnikowia pulcherrima</i> is its strong antimicrobial activity against phytopathogens, which makes it a suitable candidate for use in biocontrol during crop cultivation. However, the mechanisms of its antimicrobial activity are not currently w...
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2025-08-01
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| author | Zofia Perek Sumi Krupa Joanna Nizioł Dorota Kręgiel Tomasz Ruman Beata Gutarowska |
| author_facet | Zofia Perek Sumi Krupa Joanna Nizioł Dorota Kręgiel Tomasz Ruman Beata Gutarowska |
| author_sort | Zofia Perek |
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| description | One of the most important features of <i>Metschnikowia pulcherrima</i> is its strong antimicrobial activity against phytopathogens, which makes it a suitable candidate for use in biocontrol during crop cultivation. However, the mechanisms of its antimicrobial activity are not currently well understood. In this study, we used metabolomic methods to investigate the possible mechanisms of antimicrobial activity by <i>M. pulcherrima</i> against phytopathogenic fungi. First, we tested the antimicrobial activity of five selected isolates against eleven phytopathogenic molds. Based on the results, selected yeast–pathogen co-cultures were cultivated on liquid and solid media. The supernatants from the liquid co-cultures were analyzed using the UHPLC-QToF-UHRMS and MS/MS methods. Co-culture growth on solid agar media was examined using the LARAPPI/CI MSI method. The yeast exhibited strong antagonism toward the mold phytopathogens. The LARAPPI/CI MSI method revealed the presence of various compounds with potential antifungal activity. The complex UHPLC-QToF-UHRMS analysis confirmed that the metabolic response of <i>M. pulcherrima</i> depends on specific yeast–pathogen interactions. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-08-01 |
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| spelling | doaj-art-9f96b510e18847609dc6ff86c0bdfe752025-08-20T03:36:22ZengMDPI AGMolecules1420-30492025-08-013015326810.3390/molecules30153268Metabolomic Insights into the Antimicrobial Effects of <i>Metschnikowia</i> Yeast on PhytopathogensZofia Perek0Sumi Krupa1Joanna Nizioł2Dorota Kręgiel3Tomasz Ruman4Beata Gutarowska5Department of Environmental Biotechnology, Lodz University of Technology, Wolczanska 171/173, 90-530 Lodz, PolandFaculty of Chemistry, Rzeszow University of Technology, Powstancow Warszawy 6, 35-959 Rzeszow, PolandFaculty of Chemistry, Rzeszow University of Technology, Powstancow Warszawy 6, 35-959 Rzeszow, PolandDepartment of Environmental Biotechnology, Lodz University of Technology, Wolczanska 171/173, 90-530 Lodz, PolandFaculty of Chemistry, Rzeszow University of Technology, Powstancow Warszawy 6, 35-959 Rzeszow, PolandDepartment of Environmental Biotechnology, Lodz University of Technology, Wolczanska 171/173, 90-530 Lodz, PolandOne of the most important features of <i>Metschnikowia pulcherrima</i> is its strong antimicrobial activity against phytopathogens, which makes it a suitable candidate for use in biocontrol during crop cultivation. However, the mechanisms of its antimicrobial activity are not currently well understood. In this study, we used metabolomic methods to investigate the possible mechanisms of antimicrobial activity by <i>M. pulcherrima</i> against phytopathogenic fungi. First, we tested the antimicrobial activity of five selected isolates against eleven phytopathogenic molds. Based on the results, selected yeast–pathogen co-cultures were cultivated on liquid and solid media. The supernatants from the liquid co-cultures were analyzed using the UHPLC-QToF-UHRMS and MS/MS methods. Co-culture growth on solid agar media was examined using the LARAPPI/CI MSI method. The yeast exhibited strong antagonism toward the mold phytopathogens. The LARAPPI/CI MSI method revealed the presence of various compounds with potential antifungal activity. The complex UHPLC-QToF-UHRMS analysis confirmed that the metabolic response of <i>M. pulcherrima</i> depends on specific yeast–pathogen interactions.https://www.mdpi.com/1420-3049/30/15/3268<i>Metschnikowia pulcherrima</i> yeastantimicrobial activitymetabolomicsphytopathogens |
| spellingShingle | Zofia Perek Sumi Krupa Joanna Nizioł Dorota Kręgiel Tomasz Ruman Beata Gutarowska Metabolomic Insights into the Antimicrobial Effects of <i>Metschnikowia</i> Yeast on Phytopathogens Molecules <i>Metschnikowia pulcherrima</i> yeast antimicrobial activity metabolomics phytopathogens |
| title | Metabolomic Insights into the Antimicrobial Effects of <i>Metschnikowia</i> Yeast on Phytopathogens |
| title_full | Metabolomic Insights into the Antimicrobial Effects of <i>Metschnikowia</i> Yeast on Phytopathogens |
| title_fullStr | Metabolomic Insights into the Antimicrobial Effects of <i>Metschnikowia</i> Yeast on Phytopathogens |
| title_full_unstemmed | Metabolomic Insights into the Antimicrobial Effects of <i>Metschnikowia</i> Yeast on Phytopathogens |
| title_short | Metabolomic Insights into the Antimicrobial Effects of <i>Metschnikowia</i> Yeast on Phytopathogens |
| title_sort | metabolomic insights into the antimicrobial effects of i metschnikowia i yeast on phytopathogens |
| topic | <i>Metschnikowia pulcherrima</i> yeast antimicrobial activity metabolomics phytopathogens |
| url | https://www.mdpi.com/1420-3049/30/15/3268 |
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