Optimization and Validation of Universal Real-Time RT-PCR Assay to Detect Virulent Newcastle Disease Viruses

Optimization and Validation of Universal Real-Time RT-PCR Assay to Detect Virulent Newcastle Disease Viruses

Newcastle disease, caused by virulent strains of avian paramyxovirus 1 (APMV-1), occurs globally and has significant social and economic impact. APMV-1 is a rapidly evolving RNA virus and is genetically divided into class I and class II with almost all virulent viruses being of class II. The conside...

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Bibliographic Details
Main Authors: Ellen Ruth Alexander Morris, Megan E. Schroeder, Phelue N. Anderson, Lisa J. Schroeder, Nicholas Monday, Gabriel Senties-Cue, Martin Ficken, Pamela J. Ferro, David L. Suarez, Kiril M. Dimitrov
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Viruses
Subjects:
APMV-1
Newcastle disease
rRT-PCR
qPCR
genotype
NDV
Online Access:https://www.mdpi.com/1999-4915/17/5/670
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