Standardization and Evaluation of the LAMP Technique for the Diagnosis of Canine Visceral Leishmaniasis in Conjunctival Swab Samples Using DNA Extracted by a Silica Column and Boiling

Standardization and Evaluation of the LAMP Technique for the Diagnosis of Canine Visceral Leishmaniasis in Conjunctival Swab Samples Using DNA Extracted by a Silica Column and Boiling

The diagnosis of canine visceral leishmaniasis (CVL) presents a challenge due to a variety of non-specific clinical signs. The available tests have low sensitivity. This study aimed to standardize and evaluate the loop-mediated isothermal amplification technique with K26 target (K26-LAMP) for diagno...

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Main Authors: Isabela C. S. Santos, Daniel M. Avelar, Luciana F. C. Miranda, Cintia X. de Mello, Lucas Keidel, Maria Inês F. Pimentel, Luanna S. Ventura, Aline Fagundes, Fernanda N. Santos, Liliane F. A. Oliveira, Shanna A. Santos, Sandro Antonio Pereira, Rodrigo C. Menezes, Andreza P. Marcelino
Format: Article
Language:English
Published: MDPI AG 2024-11-01
Series:Tropical Medicine and Infectious Disease
Subjects:
canine visceral leishmaniasis
molecular biology
diagnosis
conjunctival swab
Online Access:https://www.mdpi.com/2414-6366/9/11/277
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