Comparative Study of Regulatory T Cell Function of Human CD25+CD4+ T Cells from Thymocytes, Cord Blood, and Adult Peripheral Blood
CD25+CD4+ regulatory T cells suppress T cell activation and regulate multiple immune reactions in in vitro and in vivo studies. To define the regulatory function of human CD25+CD4+ T cells at various stages of maturity, we investigated in detail the functional differences of CD25+CD4+ T cells from t...
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| Format: | Article |
| Language: | English |
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Wiley
2008-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2008/305859 |
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| author | Wakae Fujimaki Nozomu Takahashi Kei Ohnuma Masayoshi Nagatsu Hiromi Kurosawa Satoko Yoshida Nam H. Dang Takehiko Uchiyama Chikao Morimoto |
| author_facet | Wakae Fujimaki Nozomu Takahashi Kei Ohnuma Masayoshi Nagatsu Hiromi Kurosawa Satoko Yoshida Nam H. Dang Takehiko Uchiyama Chikao Morimoto |
| author_sort | Wakae Fujimaki |
| collection | DOAJ |
| description | CD25+CD4+ regulatory T cells suppress T cell activation and regulate multiple immune reactions in in vitro and in vivo studies. To define the regulatory function of human CD25+CD4+ T cells at various stages of maturity, we investigated in detail the functional differences of CD25+CD4+ T cells from thymocytes, cord blood (CB), and adult peripheral blood (APB). CB CD25+CD4+ T cells displayed low-FOXP3 protein expression level and had no suppressive activity. In contrast, CD25+CD4+ T cells from thymocytes or APB expressed high expression level of FOXP3 protein associated with significant suppressive activity. Although CB CD25+CD4+ T cells exhibited no suppressive activity, striking suppressive activity was observed following expansion in culture associated with increased FOXP3 expression and a shift from the CD45RA+ to the CD45RA− phenotype. These functional differences in CD25+CD4+ T cells from Thy, CB, and APB hence suggest a pathway of maturation for Treg in the peripheral immune system. |
| format | Article |
| id | doaj-art-9c85b2e4059c466e88be3314b5974f82 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2008-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-9c85b2e4059c466e88be3314b5974f822025-02-03T06:06:24ZengWileyClinical and Developmental Immunology1740-25221740-25302008-01-01200810.1155/2008/305859305859Comparative Study of Regulatory T Cell Function of Human CD25+CD4+ T Cells from Thymocytes, Cord Blood, and Adult Peripheral BloodWakae Fujimaki0Nozomu Takahashi1Kei Ohnuma2Masayoshi Nagatsu3Hiromi Kurosawa4Satoko Yoshida5Nam H. Dang6Takehiko Uchiyama7Chikao Morimoto8Division of Clinical Immunology, Advanced Clinical Research Center, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, JapanDivision of Clinical Immunology, Advanced Clinical Research Center, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, JapanDivision of Clinical Immunology, Advanced Clinical Research Center, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, JapanDepartment of Cardiovascular Surgery, Tokyo Women's Medical University, Tokyo 162-8666, JapanDepartment of Cardiovascular Surgery, Tokyo Women's Medical University, Tokyo 162-8666, JapanDepartment of Obstetrics and Gynecology, Yoshida Clinic, Saitama 358-0054, JapanDepartment of Hematologic Malignancies, Nevada Cancer Institute, Las Vegas, NV 89135, USADepartment of Microbiology and Immunology, Tokyo Women's Medical University, Tokyo 162-8666, JapanDivision of Clinical Immunology, Advanced Clinical Research Center, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, JapanCD25+CD4+ regulatory T cells suppress T cell activation and regulate multiple immune reactions in in vitro and in vivo studies. To define the regulatory function of human CD25+CD4+ T cells at various stages of maturity, we investigated in detail the functional differences of CD25+CD4+ T cells from thymocytes, cord blood (CB), and adult peripheral blood (APB). CB CD25+CD4+ T cells displayed low-FOXP3 protein expression level and had no suppressive activity. In contrast, CD25+CD4+ T cells from thymocytes or APB expressed high expression level of FOXP3 protein associated with significant suppressive activity. Although CB CD25+CD4+ T cells exhibited no suppressive activity, striking suppressive activity was observed following expansion in culture associated with increased FOXP3 expression and a shift from the CD45RA+ to the CD45RA− phenotype. These functional differences in CD25+CD4+ T cells from Thy, CB, and APB hence suggest a pathway of maturation for Treg in the peripheral immune system.http://dx.doi.org/10.1155/2008/305859 |
| spellingShingle | Wakae Fujimaki Nozomu Takahashi Kei Ohnuma Masayoshi Nagatsu Hiromi Kurosawa Satoko Yoshida Nam H. Dang Takehiko Uchiyama Chikao Morimoto Comparative Study of Regulatory T Cell Function of Human CD25+CD4+ T Cells from Thymocytes, Cord Blood, and Adult Peripheral Blood Clinical and Developmental Immunology |
| title | Comparative Study of Regulatory T Cell Function of Human CD25+CD4+ T Cells from Thymocytes, Cord Blood, and Adult Peripheral Blood |
| title_full | Comparative Study of Regulatory T Cell Function of Human CD25+CD4+ T Cells from Thymocytes, Cord Blood, and Adult Peripheral Blood |
| title_fullStr | Comparative Study of Regulatory T Cell Function of Human CD25+CD4+ T Cells from Thymocytes, Cord Blood, and Adult Peripheral Blood |
| title_full_unstemmed | Comparative Study of Regulatory T Cell Function of Human CD25+CD4+ T Cells from Thymocytes, Cord Blood, and Adult Peripheral Blood |
| title_short | Comparative Study of Regulatory T Cell Function of Human CD25+CD4+ T Cells from Thymocytes, Cord Blood, and Adult Peripheral Blood |
| title_sort | comparative study of regulatory t cell function of human cd25 cd4 t cells from thymocytes cord blood and adult peripheral blood |
| url | http://dx.doi.org/10.1155/2008/305859 |
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