Modulation of CCR5 expression and R5 HIV-1 infection in primary macrophages exposed to sera from HESN, LTNP, and chronically HIV-1 infected people with or without natural antibodies to CCR5
CCR5 is the main co-receptor for HIV-1 cell entry and it plays key roles in HIV-1 mucosal transmission. Natural anti-CCR5 antibodies were found in HIV-1-exposed seronegative and long-term non-progressor subjects, suggesting a role in controlling viral replication in vivo. We assessed the effect of s...
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Elsevier
2024-12-01
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| Series: | Virus Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0168170224001990 |
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| author | Iole Farina Mauro Andreotti Claudia Pastori Roberta Bona Clementina Maria Galluzzo Roberta Amici Cristina Purificato Caterina Uberti-Foppa Agostino Riva Maria Cristina Gauzzi Lucia Lopalco Laura Fantuzzi |
| author_facet | Iole Farina Mauro Andreotti Claudia Pastori Roberta Bona Clementina Maria Galluzzo Roberta Amici Cristina Purificato Caterina Uberti-Foppa Agostino Riva Maria Cristina Gauzzi Lucia Lopalco Laura Fantuzzi |
| author_sort | Iole Farina |
| collection | DOAJ |
| description | CCR5 is the main co-receptor for HIV-1 cell entry and it plays key roles in HIV-1 mucosal transmission. Natural anti-CCR5 antibodies were found in HIV-1-exposed seronegative and long-term non-progressor subjects, suggesting a role in controlling viral replication in vivo. We assessed the effect of sera containing or not natural anti-CCR5 antibodies, on membrane CCR5 level and HIV-1 infection in primary macrophages. Sera modulated CCR5 expression with a trend dependent on the donor/serum tested but independent on the presence or absence of anti-CCR5 antibodies. All sera strongly reduced HIV-1 DNA in all donor's macrophages and no correlation was observed between CCR5 and viral DNA levels. These results suggest that CCR5 expression level is not a major determinant of macrophage infection and that the observed modulation of CCR5 and HIV-1 DNA might depend on factors other than CCR5-reactive antibodies present in sera and/or intrinsic to the donors on which sera were tested. |
| format | Article |
| id | doaj-art-9c651366ecdf46aab31afe7fda981cd1 |
| institution | OA Journals |
| issn | 1872-7492 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Virus Research |
| spelling | doaj-art-9c651366ecdf46aab31afe7fda981cd12025-08-20T01:58:08ZengElsevierVirus Research1872-74922024-12-0135019950610.1016/j.virusres.2024.199506Modulation of CCR5 expression and R5 HIV-1 infection in primary macrophages exposed to sera from HESN, LTNP, and chronically HIV-1 infected people with or without natural antibodies to CCR5Iole Farina0Mauro Andreotti1Claudia Pastori2Roberta Bona3Clementina Maria Galluzzo4Roberta Amici5Cristina Purificato6Caterina Uberti-Foppa7Agostino Riva8Maria Cristina Gauzzi9Lucia Lopalco10Laura Fantuzzi11National Center for Global Health, Istituto Superiore di Sanità, Rome, ItalyNational Center for Global Health, Istituto Superiore di Sanità, Rome, ItalyDivision of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Milan, ItalyNational Center for Global Health, Istituto Superiore di Sanità, Rome, ItalyNational Center for Global Health, Istituto Superiore di Sanità, Rome, ItalyNational Center for Global Health, Istituto Superiore di Sanità, Rome, ItalyNational Center for Global Health, Istituto Superiore di Sanità, Rome, ItalyInfectious diseases clinic, Vita-Salute San Raffaele University, Milan, ItalyDepartment of Biomedical and Clinical Sciences, University of Milan, Milan, ItalyNational Center for Global Health, Istituto Superiore di Sanità, Rome, ItalyDivision of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy; CorrespondenceNational Center for Global Health, Istituto Superiore di Sanità, Rome, Italy; CorrespondenceCCR5 is the main co-receptor for HIV-1 cell entry and it plays key roles in HIV-1 mucosal transmission. Natural anti-CCR5 antibodies were found in HIV-1-exposed seronegative and long-term non-progressor subjects, suggesting a role in controlling viral replication in vivo. We assessed the effect of sera containing or not natural anti-CCR5 antibodies, on membrane CCR5 level and HIV-1 infection in primary macrophages. Sera modulated CCR5 expression with a trend dependent on the donor/serum tested but independent on the presence or absence of anti-CCR5 antibodies. All sera strongly reduced HIV-1 DNA in all donor's macrophages and no correlation was observed between CCR5 and viral DNA levels. These results suggest that CCR5 expression level is not a major determinant of macrophage infection and that the observed modulation of CCR5 and HIV-1 DNA might depend on factors other than CCR5-reactive antibodies present in sera and/or intrinsic to the donors on which sera were tested.http://www.sciencedirect.com/science/article/pii/S0168170224001990HIV-1Natural anti-CCR5 antibodyPrimary macrophage |
| spellingShingle | Iole Farina Mauro Andreotti Claudia Pastori Roberta Bona Clementina Maria Galluzzo Roberta Amici Cristina Purificato Caterina Uberti-Foppa Agostino Riva Maria Cristina Gauzzi Lucia Lopalco Laura Fantuzzi Modulation of CCR5 expression and R5 HIV-1 infection in primary macrophages exposed to sera from HESN, LTNP, and chronically HIV-1 infected people with or without natural antibodies to CCR5 Virus Research HIV-1 Natural anti-CCR5 antibody Primary macrophage |
| title | Modulation of CCR5 expression and R5 HIV-1 infection in primary macrophages exposed to sera from HESN, LTNP, and chronically HIV-1 infected people with or without natural antibodies to CCR5 |
| title_full | Modulation of CCR5 expression and R5 HIV-1 infection in primary macrophages exposed to sera from HESN, LTNP, and chronically HIV-1 infected people with or without natural antibodies to CCR5 |
| title_fullStr | Modulation of CCR5 expression and R5 HIV-1 infection in primary macrophages exposed to sera from HESN, LTNP, and chronically HIV-1 infected people with or without natural antibodies to CCR5 |
| title_full_unstemmed | Modulation of CCR5 expression and R5 HIV-1 infection in primary macrophages exposed to sera from HESN, LTNP, and chronically HIV-1 infected people with or without natural antibodies to CCR5 |
| title_short | Modulation of CCR5 expression and R5 HIV-1 infection in primary macrophages exposed to sera from HESN, LTNP, and chronically HIV-1 infected people with or without natural antibodies to CCR5 |
| title_sort | modulation of ccr5 expression and r5 hiv 1 infection in primary macrophages exposed to sera from hesn ltnp and chronically hiv 1 infected people with or without natural antibodies to ccr5 |
| topic | HIV-1 Natural anti-CCR5 antibody Primary macrophage |
| url | http://www.sciencedirect.com/science/article/pii/S0168170224001990 |
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